摘要
目的:观察复方泽泻滴丸对豚鼠内耳眩晕症模型的影响,为复方泽泻滴丸的新药研制提供实验依据;方法:将豚鼠60只随机分成6组,每组10只,分别为正常对照组,模型对照组,模型+阳性药物组,模型+药物高、中、低剂量组。除正常对照组外均采用进口醋酸去氨加压素腹腔注射4ug.kg-1.d-1,共7d后,将剂量加大为6ug/kg继续注射3d进行豚鼠耳蜗膜迷路积水造模,24h后对除正常对照组外的各组按方案进行给药一周后处死取材,采用耳蜗切片后应用Image-ProPlus6.0分析软件系统观察内耳组织平均中阶面积(SMA)增加率及形态学的变化来研究耳蜗膜迷路积水的变化情况,用来验证复方泽泻滴丸的药效。结果:利用SPSS14.0软件,对数据进行秩和检验:阳性对照组以及中剂量药物组和正常对照组之间比较P>0.05,组间无明显差异,说明中剂量组疗效显著,接近于阳性药物的疗效,治愈到接近正常状态。高剂量药物组和正常对照组比较组间有差异0.01<P<0.05,说明高剂量组有效但疗效不及中剂量组明显,治疗到正常状态还有一定差距。模型组及低剂量药物和正常对照组比较组间具有显著性差异P<0.01,说明低剂量组疗效微弱用量不足,接近未经治疗的模型组的状态,和正常状态有明显差异。结论:中剂量复方泽泻滴丸对内耳眩晕症有明显的治疗效果。
Objective：To observe the effects of the Fufang Zexiedi pills against guinea pig inner ear vertigo Model.Methods：60 cases were randomly divided into six groups,10 for each,respectively for normal control group,Model control group,Model＋positive drug group,Model ＋ drug high,Medium and low dose group.Imported acetate desmopressin abdominal injection 4ug/kg/d were given except normal control group;7 days later,The dose was increased to 6ug/kg,continue injection three days,guinea pig cochlea endolymphatic modeling,Each group were sacrificed by program In addition to the normal control group after 24 hours,observation SMA rate of increase and morphological changes to study the changes of the cochlear endolymphatic used to verify the efficacy of the Fufang Zexiedi pills.Use SPSS14.0 software,normal control group,control group and positive high dose drugs were compared P 0.05;high dose group and normal control group were compared,the differences were significant 0.01P0.05;the differences were significant P0.01 in model control group,low dose group and normal control group.Conclusion：Medium dose of the Fufang Zexiedi pills had significant therapeutic effect in treating vertigo inner ear disease.
出处
《中医临床研究》
2012年第5期37-41,共5页
Clinical Journal Of Chinese Medicine
基金
福建省科技厅科研课题支助项目(项目编号:2008Y0046)
福建省自然科学基金项目(项目编号:2009J01166)
关键词
复方泽泻滴丸
药效学
动物模型
耳蜗膜迷路积水
Fufang Zexiedi pills
Pharmacodynamics
Animal models
Cochlear endolymphatic
