摘要
在成功地抽提出国际公认在诱导血吸虫保护性免疫力上起重要作用的24-26kD和90kD蛋白质“靶抗原”的基础上,进一步应用上述抗原免疫小鼠,经融合试验及克隆化获得分泌抗日本血吸虫蛋白质“靶抗原”单克隆抗体(McAb)的杂交瘤细胞29株,其中IgG类16株(IgG_1 7株,IgG_(2a) 5株,IgG_(2b) 1株,IgG_3 3株),IgM 13株。以后,又从McAb的定位研究、McAb体外ADCC对血吸虫童虫的杀伤效应、McAb在不同血吸虫抗原中的识别位点(抗原结合位)等方面,对报获的McAb进行了研究。
In the studies presented here, we demonstrated the feasibility of producing large number of hybridoma cell lines secreting McAbs against S. japonicum including 16 cell lines secreting IgG McAbs (7 for IgG1, 5 for IgG2a, 1 for IgG2b, 3 for IgG3) and 13 McAbs for IgM, on the basis of successfully extracting 24-26kD and 90kD 'target an tigen' proteins known as important antigens for inducing schistosome protective immunity All the above cell lines were characterized for localization of the McAbs, mediating in vitro ADCC against schistosomula, epitope recognized by the McAbs on different kinds of schistosome antigens. Studies have shown the evidences that the McAbs can be used to identify the 'target antigen' on the surface of schistosome, to isolate and purify 'target antigen' of S. japonicum by chromatography column bearing McAbs, as well as to immunize animals as antigens for accumulation of data in the development of vaccine against S. japonicum via anti-idiotype antibodies.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1990年第2期88-91,共4页
Chinese Journal of Parasitology and Parasitic Diseases
基金
国家科委863高技术计划资助~~
关键词
血吸虫
单克隆抗体
靶抗原
Schistosoma japopicum, protein 'target antigen', monoclonal antibody
