摘要
建立了呋喃唑酮代谢物AOZ、呋喃西林代谢物SEM、呋喃妥因代谢物AHD、呋喃它酮代谢物AMOZ的高效液相色谱一串联质谱（HPLC—MS／MS）测定方法。样品经乙酸乙酯提取后，用OasisHLB固相萃取柱净化，以乙腈和甲酸一乙酸铵缓冲溶液为流动相，梯度洗脱，采用电喷雾正离子（ESI＋）电离，多反应监测（MRM）模式检测，外标法定量。4种硝基呋喃类代谢物在0．5-20．0ng／mL范围内均具有良好的线性关系，相关系数不低于0．9906，平均加标回收率为91．O％～101．5％，相对标准偏差均小于8％。该方法具有准确、灵敏、高效、环保，适用于猪肝中多种硝基呋喃类代谢物的同时测定。
A HPLC-MS/MS method was developed for determination of 4 nitrofuran metabolite residues in liver. The sample was extracted with ethyl acetate and purified by Oasis HLB solid-phase extraction column, then detected by high performance liquid chromatography tandem mass spectrometry （HPLC-MS/MS）. Acetonitrile and formic acid-ammonium acetate buffer were used as mobile phase by gradient elution. The analysis of target compounds was carried out by HPLC-MS/MS under positive eleetrospray ionization （ESI~） and multiple reaction monitoring （MRM） mode. The calibration curves for 4 nitrofuran metabolite residues all had good linear relationships with correlation coefficients not less than 0.9906. The spiked recoveries were ranged from 91.0% to 101.5% with relative standard deviations less than 8%. It was an effective method which fit for the nitrofuran metabolite residues analysis and has high accuracy, precision and sensitivity.
出处
《广东农业科学》
CAS
CSCD
北大核心
2012年第4期87-89,共3页
Guangdong Agricultural Sciences
基金
中山市科技计划项目(20114A195)
