摘要
采用PCR扩增和DNA直接测序技术,测定分析了采自珠江水系西江的30尾稀有白甲鱼(Onychostoma rara)mtDNA D环约467 bp的序列。结果显示,该序列的长度为467～470 bp,共计发现了16个变异位点,占分析总位点数的3.40%,其碱基组成A+T的平均含量(67.6%)高于C+T(32.3%)。稀有白甲鱼西江种群mtDNA D环区在结构上可分为终止序列区、中央保守区和保守序列区,存在着与终止相关的序列ETAS以及保守序列CSB-F、CSB-E、CSB-D和CSB1。定义了11种单倍型,单倍型多样性指数(Hd)为0.733 3,单倍型之间平均遗传距离(P)为0.010 2,核苷酸多样性(Pi)为0.005 79,平均核苷酸差异数(K)为2.705 75。11个单倍型构建的非加权分组平均法(UPGMA)系统树聚为2个分支。该研究表明稀有白甲鱼西江种群存在着较丰富的遗传多样性。研究亮点:(1)有关濒危鱼类稀有白甲鱼西江种群mtDNA D环测序及遗传多样性研究尚未见报道;(2)本研究揭示了稀有白甲鱼西江种群尚保存有较丰富的遗传多样性;(3)对稀有白甲鱼西江种群遗传学研究具有理论价值,并对开展该种群的保护和资源利用具有现实指导意义。
The genetic diversity of mtDNA D-loop in the population of Onychostoma rara from the Xijiang River,the Pearl River system was studied for the first time on the basis of determination of about 467 bp sequences of mtDNA D-loop in 30 individuals by the methods of PCR and DNA sequencing.The results showed that the length of the determined sequence in 30 individuals varied from 467 to 470 bp.A total of 16 variable loci（3.40% of total loci） were detected in the sequence of mtDNA D-loop in the population,and the total percentage of bases A and T（67.6%） was much higher than that of bases C and G（32.3%） in the sequence.The mtDNA D-loop of Onychostoma rara comprised the termination associated sequence domain,the central conserved sequence domain and the conserved sequence block domain,and the extended termination associated sequence（ETAS）,3 conserved blocks（CSB-F,CSB-E,CSB-D） in the central conserved sequence domain and 1 conserved sequence blocks（CSB1） in the conserved sequence block domain were successfully identified in the population.The 30 individuals belonged to 11 haplotypes according to the determined sequences.The haplotype diversity（Hd） was 0.733 3,and the average genetic distance（P） between the haplotypes of the population was 0.010 2.The nucleotide diversity（Pi） of the 30 individuals was 0.005 79,and the average number of nucleotide differences（K） of them was 2.705 75.The UPGMA phylogenetic tree of the 11 haplotypes comprised 2 branches.The population of O.rara from the Xijiang River possessed abundant genetic diversity.
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2012年第2期176-182,共7页
Journal of Shanghai Ocean University
基金
国家自然科学基金(30760189
30960297)
贵州省科学技术基金[黔科合J字(2007)2062]
贵州省省长基金[黔省专合字(2005)347]
