摘要
精子发生是男性生殖中的主要过程,精原细胞的不断分裂增殖又保证了精子发生的顺利进行。随着年龄的不断增长,男性精子的数量、质量出现下降趋势。mTOR信号转导通路在细胞增殖分化中发挥着中心调控作用,因此,mTOR信号通路可能在精子发生过程中有着重要的地位。为了探明mTOR信号通路与精子发生的关系,首先,通过SD大鼠睾丸组织切片的免疫组化,发现mTOR是在生精小管的精原细胞胞浆中表达;其次,采用FQ-PCR检测mTOR mRNA在SD大鼠睾丸中的表达。结果显示,80周龄组mTOR的转录与8周龄组相比差异显著。最后利用Western blot检测出mTOR蛋白的表达及其对下游靶蛋白P70S6K的磷酸化效率均随年龄的增长逐渐下降。同时,在用雷帕霉素处理8周龄SD大鼠中,发现精子数量减少,P70S6K磷酸化效率降低并伴随生精小管萎缩和空泡化。通过这些结果,可以看出mTOR信号转导通路可能在精子发生中发挥着重要作用。
permatogenesis was very important in male reproductive and the proliferate of spermatogonia ensure the spermatogenesis successfully.As the growth of the age,the quantity and quality of sperm reduce gradually.mTOR signaling pathway played a central regulatory role in cell proliferation and differentiation.Therefore,mTOR signaling pathway played an important role in spermatogenesis probably.To investigate the relationship between the mTOR signaling pathway and spermatogenesis,we did the SD rat testis tissue sections by immunohistochemistry and we found mTOR expressed in the spermatogonia.Then,we detected the expression of mTOR mRNA in the different developmental stages of rat testis by FQ-PCR,and the results showed the significant differences of transcription between 80 weeks group and 8 weeks group.At last,we found the expression of mTOR and the phosphorylation efficiency of P70S6K reduced gradually as the growth of the age by Western blot.After treated with rapamycin,the P-P70S6K/P70S6K and the number of sperm reduced in the normal 8 weeks SD rat.These results convinced us that mTOR signaling pathway was very important in the spermatogenesis.
出处
《中国细胞生物学学报》
CAS
CSCD
北大核心
2012年第3期218-225,共8页
Chinese Journal of Cell Biology
基金
重庆医科大学重点基金(No.XBED201007)资助项目~~
