摘要
目的观察5-FU对TRAIL诱导的胃癌BGC823细胞凋亡的影响,明确死亡受体5(DR5)在5-FU和TRAIL诱导凋亡中的作用。方法采用MTT法测定细胞活力、流式细胞仪检测细胞凋亡、免疫印迹检测蛋白表达。结果 TRAIL可导致BGC823细胞轻度的增殖抑制和少量的细胞凋亡。与单药TRAIL和5-FU相比,TRAIL联合5-FU对细胞的增殖抑制和诱导凋亡作用明显增强(P<0.05)。免疫印迹结果显示,TRAIL没有改变DR5的蛋白表达,而5-FU作用BGC823细胞48 h后,DR5蛋白表达上调(P<0.05)。TRAIL和5-FU联合作用后,DR5蛋白表达同样明显上调(P均<0.05)。结论 5-FU通过上调DR5蛋白表达提高了BGC823细胞对TRAIL的敏感性。
To observe the inhibition effect of 5-FU on tumor necrosis factorrelated apoptosisinducing ligand （TRAIL）induced apoptosis in gastric cancer BGC823 cells, and identify the action of death receptor 5 （DR.5） in 5-FU and TRAILinduced apoptosis. Methods Cell proliferation was measured using MTF assay. Cell apoptosis was determined by flow cytometry. Expression of protein was analyzed by western blot. Results Treatment with 100 ng/mL TRAIL for 48 h slightly resulted in the inhibition of cell proliferation and a little cell apoptosis in BGC823 cells. Treatment with TRAIL （100 ng/mL） and 5-FU （5.55 g/mL, IC50 dose of 48 h） leaded to a dramatic increase in the inhibitiong of cell proliferation and cell apoptosis compared to treatment with 5-FU or TRAIL alone （ P 〈 0.05 ）. TRAIL alone did not change the ex pression of DR.5, while 5.55 g/mL 5-FU significantly uprcgulated the expressions of DR5 in BGC823 cells after 48 h （ P 〈 0.05 ）. Treatment with 5-FU and TRAIL also resulted in the upregulation of DR5 （ all P 〈 0.05 ）. Conclusion 5-FU en- hance TRAIL-induced apoptosis in gastric cancer BGC823 celis by the upregulation of DRS.
出处
《山东医药》
CAS
2012年第1期1-3,共3页
Shandong Medical Journal
基金
高等学校博士学科点专项科研基金(20102104120008)
中国医科大学附属第一医院科学研究基金(fsfh1003)
