摘要
背景:软骨寡聚基质蛋白(cartilage oligomeric matrix protein,COMP)是软骨中非胶原蛋白的主要成分,软骨的损伤、修复和代谢变化均可能影响COMP的表达水平。目的:研究骨关节炎(osteoarthritis,OA)患者关节滑液中COMP水平与病变严重程度的相关性,探讨注射玻璃酸钠对关节滑液中COMP的影响。方法:52例膝关节OA患者接受关节内注射玻璃酸钠治疗,治疗前、治疗后6个月行X线片检查,按Kellgren放射学诊断标准评级,记录治疗前和治疗开始后5周、6个月时患者膝关节的WOMAC评分。采用ELISA方法测定在接受透明质酸钠治疗前、治疗开始后5周关节液中的COMP水平。19例因半月板或韧带损伤接受关节镜手术的患者作为正常对照组。结果:OA组患者滑液中COMP水平明显高于对照组,有统计学差异(P=0.036)。OA组患者滑液中COMP水平与OA严重程度(WOMAC评分)呈正相关(P=0.001),与影像学Kellgren分级标准无相关性(P=0.12)。结论:滑液中COMP水平与OA病变严重程度呈正相关,测定OA患者血中COMP水平对进一步研究OA发病机理、早期发现并采取有效防治策略及监测防治效果具有极其重要的意义。
Background： Cartilage oligomeric matrix protein （COMP） is the main component of cartilage non-collagenous protein. Cartilage injury, repair and metabolic changes that may affect the expression level of COMP.Objective： To investigate the relationship between the levels of COMP in synovial fluid and severity of osteoarthritis Methods： A total of 52 patients with knee osteoarthritis received intra-articular injections of sodium hyaluronate and were followed up for 6 months. WOMAC score was recorded before treatment, 5 weeks and 6 months after treatment. Radio- graphic findings were evaluated according to the Kellgren-Lawrence grade before treatment and 6 months after treatment. The levels of COMP in synovial fluid were analyzed by enzyme-linked immunosorbent assay （ELISA）. Synovial fluid of knee joint was collected from 19 controls （ meniscus or ligament injury without cartilage injury under arthroseopy）.Results： The levels of COMP in osteoarthritis patients were significantly higher than those in control group （ P = 0. 036 ）. The levels of COMP in synovial fluid was positively correlated with WOMAC score （ P = 0. 001 ） , but not with Kellgren- Lawrence grade （P =0. 12）. Conclusions ： The level of COMP in synovial fluid is related with the severity of osteoarthritis. COMP is a sensitive marker reflecting the metabolic changes of articular cartilage.
出处
《中国骨与关节外科》
2012年第1期60-64,共5页
Chinese Journal of Bone and Joint Surgery
关键词
骨关节炎
软骨寡聚基质蛋白
酶联免疫吸附测定
osteoarthritis
cartilage oligomeric matrix protein
enzyme-linked immunosorbent assay
