摘要
目的建立测定牧马豆药材不同部位金雀花碱的RP-HPLC的方法。方法以Agilent C_(18)色谱柱(250 mm×4.6 mm,5μm)为分析柱,乙腈-0.02%醋酸铵-三乙胺(15:85:0.02)为流动相,检测波长230 nm,柱温40℃。结果进样量在0.35～1.75μg范围,金雀花碱峰面积与浓度呈良好线性关系(r=0.999 9),回收率98.2%(n=6)。结论此法简便、快速、准确,可用于测定该药材中金雀花碱的含量。
Objective To establish a RP-HPLC method for the determination of cytisine in different parts of lanceleaf thermopsis herb. Methods The Agilent C18 column （250 mm × 4.6 mm） was used with the mixture of acetonitrile -0.02% CHaCOONH4-triethylamine （TEA） （15： 85. 0.02） as the mobile phase. The detection wavelength was set at 230 nm and the column temperature was set at 40℃. Results A good linear relationship between peak area and concentration of cytisine was achieved in the range of 0.35 - 1.75μg （r=0.9999）. The average recovery of cytisine was 98.2% （n=6）. Conclusion This method is simple, rapid, accurate and can be used for the determination of cytisine in different parts of lanceleaf thermopsis herb.
出处
《食品与药品》
CAS
2012年第2期123-125,共3页
Food and Drug
关键词
牧马豆
金雀花碱
HPLC
lanceleafthermopsis herb
cytisine
HPLC
