摘要
目的应用基因重组技术在大肠杆菌中高效表达藤黄微球菌过氧化氢酶(catalase,CAT)。方法从藤黄微球菌DNA中获得CAT编码基因,并应用pProEx-HTa质粒构建融合表达载体并表达和检测活性。结果通过融合表达获得可溶性带6×His标签重组蛋白,该蛋白经过Ni-NTA纯化后可获得活性物质。结论从大肠杆菌表达体系中表达了具有生物活性的CAT。
Objective To produce Micrococcus luteus catalase （CAT） on a large scale in E. coli by recombinant DNA technology. Methods CAT gene was obtained by PCR from Micrococcus luteus DNA, and the expression vectors were constructed by using pProEx-HTa plasmids and transformed into E. coli. Results The fusion expressed recombination proteins were soluble and labeled with 6 × His. By Ni-NTA affinity chromatography, the active CAT was obtained from the recombination proteins. Conclusion The recombinant Micrococcus luteus CAT with biological activity can be obtained from E. coli expression system.
出处
《食品与药品》
CAS
2012年第2期81-84,共4页
Food and Drug
基金
上海市科委纳米技术专项(1052nm05702)
