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整合子与大肠埃希菌耐药性的相关性研究

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摘要 目的调查大肠埃希菌中Ⅰ类、Ⅱ类和Ⅲ类整合子的存在情况,分析细菌携带的整合子与大肠埃希菌耐药性的关系。方法测定87株大肠埃希菌对抗菌药物的敏感性;应用简并引物聚合酶链反应(PCR)方法,同时扩增整合子5′保守区的Ⅰ类、Ⅱ类和Ⅲ类整合酶基因,对阳性PCR产物用限制性内切酶HinfⅠ作限制片段长度多态性(RFLP)分析进行整合子分类。结果 30株大肠埃希菌检测出Ⅰ类整合子,未检出Ⅱ类和Ⅲ类整合子。Ⅰ类整合子阳性菌株对抗菌药物的耐药率普遍比整合子阴性菌株高。结论大肠埃希菌临床分离株的耐药性强,细菌的多重耐药与Ⅰ类整合子相关。
出处 《检验医学与临床》 CAS 2012年第6期694-696,共3页 Laboratory Medicine and Clinic
基金 福建省卫生厅青年科研基金(39107)
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  • 1Severino P,Magalhaes VD.The role of integrons in thedissemination of anti-biotic resistance among clinical iso-lates of Pseudomonas aeruginosa from an intensive care u-nit in Brazil[J].Res Microbiol,2002,153(4):221-226.
  • 2夏涵,府伟灵,陈鸣,黄庆,赵猛,赵渝徽,王丰,罗阳.快速提取细菌DNA方法的研究[J].现代预防医学,2005,32(5):571-573. 被引量:72
  • 3White PA,McIver CJ,Rawlinson WD.Integrons and genecassettes in the entero-bacteriaceae[J].Antimicrob A-gents Chemother,2001,45(9):2658-2661.
  • 4Levesque C,Piche L,Larose C,et al.PCR mapping of in-tegrons reveals several novel combinations of resistancegenes[J].Antimicrob Agents Chemother,1995,39(1):185-191.
  • 5Daly M,Buckley J,Power E,et al.Molecular characteriza-tion of Irish Salmonella enterica serotype typhimurium:detection of class I integrons and assessment of gene-ticrelationships by DNA amplification fingerprinting[J].Ap-pl Environ Microbiol,2000,66(2):614-619.
  • 6Didier M,Broderick D,Vera A,et al.Antibiotic Resist-ance in the ECOR Collection:Integrons and Identificationof a Novel aad Gene[J].Antimicrob Agents Chemother,2000,44(6):1568-1574.
  • 7Sotto A,De Boever CM,Fabbro-Peray P,et al.Risk fac-tors for antibiotic-resistant Escherichia coli isolated fromhospitalized patients with urinary tract infections:apro-spectives tudy[J].J Clin Microbiol,2001,39(2):438-444.
  • 8杜艳,郭翀,胡莹,陈端.整合子介导的大肠埃希菌临床菌株多重耐药研究[J].临床检验杂志,2005,23(2):88-90. 被引量:18
  • 9Martinez-Freijo P,Fluit AC,Schmitz FJ,et al.Class I in-tegrons in Gram-negative isolates from different Europeanhospitals and association with decreased susceptibility tomultiple antibiotic compounds[J].J Antimicrob Chemoth-er,1998,42:689-696.
  • 10吴劲松,卢月梅,吴伟元,李文青.尿标本来源大肠埃希菌Ⅰ、Ⅱ类整合子检测及基因分型研究[J].国际检验医学杂志,2011,32(12):1281-1282. 被引量:3

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