摘要
采用排阻色谱-多角度激光光散射-折光联用技术(SEC-MALLS-RI)研究高静压处理(HP)对芸豆分离蛋白分子量分布的影响规律,揭示HP导致KPI聚集-解离行为。根据Debye plot方法,计算出芸豆球蛋白的绝对分子量为161kDa,200MPa HP处理解离KPI的可溶性聚集物,而400和600MPa HP处理诱导不溶性聚集物向可溶性聚集物转化,改善KPI的溶解度(PS)。
The influence of high pressure （HP） treatment （200-600 MPa） on molecular distribution of kidney bean （Phaseolus vulgaris L） protein isolate （KPI） was investigated by High-performance size-exclusion chromatography combined with multi-angle laser light seattering technique （HPSEC-MALLS）, to explore the possible HP-induced protein association-dissociation behavior. The molecular weight （Mw） of phaseolin, the major component in KPI, calculated based on the Debye plot, was about 161 kDa. HP treatment at 200 MPa led to dissociation of soluble aggregates （at void volume）, while HP treatment at 400 and 600 MPa led to dissociation of insoluble aggregates in KPI, thus improved PS.
出处
《现代食品科技》
EI
CAS
北大核心
2012年第3期252-255,共4页
Modern Food Science and Technology
基金
广东省自然科学基金资助项目(10451064101005149)
关键词
芸豆分离蛋白
聚集
解离
高静压
溶解度
kidney bean （Phaseolus vulgaris L.） protein
association
dissocation
high pressure
solubility
