摘要
目的:研究食管癌发病过程中染色质解旋酶DNA结合蛋白5(Chromodomain helicase DNA-binding protein5,CHD5)基因表观遗传学改变,探讨CHD5基因甲基化作为食管癌诊断标志物的可行性.方法:用甲基化特异性聚合酶链反应(methylation specific PCR,MSP)检测72例食管癌组织及成对癌旁组织,9例正常食管黏膜组织及4株食管癌细胞系中CHD5基因的甲基化状态,并用逆转录聚合酶链式反应(RT-PCR)检测CHD5基因在上述食管癌细胞系的表达.结果:69%(50/72)食管癌组织发生CHD5基因启动子区甲基化,32%(23/72)癌旁组织发生甲基化,差异具有统计学意义(χ2=20.254,P<0.05).9例食管正常组织未发生甲基化,2株食管癌细胞系中由于基因启动子区甲基化导致CHD5基因表达缺失,经5-aza-deoxycytidine处理96h后CHD5重新表达.结论:食管癌中CHD5基因频繁发生甲基化,表观遗传学改变是其基因表达的重要调节机制,CHD5基因甲基化有可能作为食管癌诊断的标志物.
AIM：To investigate epigenetic changes of the chromodomain helicase DNA-binding protein 5（CHD5） gene during esophageal carcinogenesis,and to explore the possibility of using CHD5 promoter methylation as a marker for human esophageal cancer.METHODS：Methylation-specific polymerase chain reaction（MSP） was used to detect the methylation status of CHD5 in 72 cases of esophageal cancer and matched tumor-adjacent tissue,9 cases of normal esophageal mucosa,and 4 esophageal cancer cell lines.Reverse transcription-polymerase chain reaction（RT-PCR） was performed to detect the expression of CHD5 in esophageal cancer cell lines mentioned above.RESULTS：CHD5 methylation was detected in 69%（50？72） of cases of esophageal cancer and 32%（23？72） of cases of matched tumor-adjacent tissue（χ2 = 20.254,P 0.05）,but not detected in 9 cases of normal esophageal mucosa.Loss of CHD5 expression was found in 2 esophagealcancer cell lines which showed CHD5 promoter methylation,and after treatment with 5-azadeoxycytidine for 96 h,CHD5 was re-expressed.CONCLUSION：CHD5 is frequently methylated in esophageal cancer.Epigenetic change may be an important mechanism for regulation of CHD5 expression,and CHD5 promoter methylation may be used as a marker for human esophageal cancer.
出处
《世界华人消化杂志》
CAS
北大核心
2012年第4期323-326,共4页
World Chinese Journal of Digestology
