摘要
目的了解从儿童或成人中分离获得的金黄色葡萄球菌(金葡菌)菌株携带Panton-Valentine杀白细胞素(PVL)基因的差异。方法分别对66株从儿童中及76株成人中分离获得的金葡菌菌株采用多重PCR,检测金葡菌16SrRNA基因、PVL基因和甲氧西林耐药决定分子A(mecA)基因;并同时检测耐甲氧西林金葡菌(MRSA)的葡萄球菌盒式染色体mec(SCCmec)基因型及亚型。结果在66株从儿童分离的金葡菌株中,共检出7株mecA基因阳性,MRSA 7/66(占10.6%);而在76株从成人分离的菌株中,共检出39株mecA基因阳性,MRSA 39/76(占51.3%),成人菌株MRSA发生率显著高于儿童(χ2=26.73,P<0.01)。从儿童或成人中分离的金葡菌菌株PVL基因携带率分别为31/66(占47.0%)和6/76(占7.9%);从儿童或成人中分离的甲氧西林敏感金葡菌(MSSA)的PVL基因携带率分别为29/59(占49.2%)和2/37(占5.4%),儿童与成人的差异均有统计学意义(P<均0.01);从儿童中分离的2株携带PVL基因的MRSA菌株都属于SCCmecⅣ型,从成人中分离的4株携带PVL基因的MRSA菌株中,SCCmecⅠ型、Ⅱ型各1株,SCCmecⅢ型2株。结论从儿童中分离的金葡菌株携带PVL基因阳性率较成人高,携带PVL基因以MSSA菌株为主。
Objective To understand the prevalence of Staphylococcus aureus carrying Panton-Valentine leukocidin gene（PVL gene）in isolates from children and adult.Methods The 16SrRNA gene,PVL gene and mecA gene of Staphylococcus aureus from 66 children＇s isolates and 76 adult isolates were determined by multiplex PCR.After that,the SCCmec genetype and subtype of methicillin-resistant Staphylococcus aureus（MRSA）were determined by the other multiplex PCR.Results Among Staphylococcus aureus strains isolated from 66 children,7 isolates were MRSA（10.6%）.Among Staphylococcus aureus strains isolated from 76 adults,39 isolates were MRSA positive（10.6%）.The positive rate of MRSA in adult was significantly higher than that in children（χ2 = 26.73,P 0.01）.The positive rate of PVL gene in children＇s isolates and adult isolates were 47.0% and 7.9% respectively.The positive rate of methicillin-sensitive Staphylococcus aureus（MSSA）carrying PVL gene in children＇s isolates and adult isolates were 49.2% and 5.4% respectively.The differences between children＇s isolates and adult isolates were all statistically significant（P 0.01）.The PVL gene in 2 children isolates was SCCmecⅣ.4 adult MRSA carried PVL gene,one was SCCmecⅠ,one was SCCmecⅡ,and the other two were SCCmecⅢ.Conclusions The rate of Staphylococcus aureus carrying PVL gene in children＇s isolates is higher than that in adult isolates.The Staphylococcus aureus carrying PVL gene in children＇s isolates is primarily MSSA.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2012年第3期251-255,共5页
Journal of Clinical Pediatrics
基金
江西省卫生厅普通科技计划项目(No.20071071)
关键词
金黄色葡萄球菌
成人
儿童
PVL基因
Staphylococcus aureus
adult
child
Panton-Valentine leukocidin gene
