摘要
2011年在西藏当雄县感染皮蝇蛆牦牛的背部皮下收集到三期幼虫113个,首先进行了形态学鉴定,之后提取DNA,用特异性引物扩增COⅠ基因,并进行测序,与GenBank中的相关序列进行了同源性比较,建立了系统发育树。结果表明,西藏当雄县感染牦牛的皮蝇蛆为牛皮蝇蛆和中华皮蝇蛆。前者(85条)占样本总数(113)的75.22%,为西藏当雄牦牛皮蝇蛆病病原的优势虫种;COⅠ基因序列显示牛皮蝇和中华皮蝇的种间差异为10.8%～11.3%,同源性为88.7%～89.2%;牛皮蝇的株间差异为0.1%～0.6%,同源性为99.4%～99.9%,中华皮蝇的株间差异为0.1%～0.4%,同源性为99.6%～99.9%。说明COⅠ基因是牦牛皮蝇蛆病病原种类分子分类鉴定的可靠靶基因。
A totol of 113 third stage larvae of Hypoderma spp.were collected at Dangxiong County of Tibet in 2011 and morphologically characterized.Cytochrome oxidaseⅠ（COⅠ） gene was amplified,sequenced and compared with COⅠ sequences of other species in the GenBank,with a phylogenetic tree constructed.Result indicated that the specimens from the County were Hypoderma bovis and H.sinence.The H.bovis was 75.22%（85/113） of total specimen and it was the prevalent species in Dangxiong County.The interspecific difference of COⅠ sequence between H.bovis and H.sinence was 10.8%-11.3%,and the homology was 88.7%-89.2%.The intraspecific difference and the homology of H.bovis was 0.1%-0.6% and 99.4%-99.9%,respectively,as well as 0.1%-0.4% and 99.6%-99.9% of H.sinence,respectively.The COⅠ was therefore confirmed to be reliable target gene for the identification of hypodermosis＇ pathogen.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2012年第3期238-242,共5页
Chinese Veterinary Science
基金
西藏自治区科技厅重点科研项目
关键词
西藏
牛皮蝇
中华皮蝇
COⅠ基因
分子分类
系统发育树
Tibet
Hypoderma bovis
Hypoderma sinence
cytochrome oxidase Ⅰ gene
molecular classification
phylogenetic tree
