摘要
采用碱性蛋白酶(Alcalase)水解豌豆蛋白,对不同条件下制备的豌豆蛋白酶水解产物DPPH.清除率进行了研究,在单因素试验基础上采用响应面分析方法优化酶水解条件,得到最佳酶解工艺条件为:酶解温度56.5℃,酶解时间3.2 h,pH6.1,加酶量3.0%,底物浓度3.0%,在此条件下,碱性蛋白酶水解产物对DPPH.的清除率为47.83%.
In this paper, we used alcalase as catalyst to hydrolyze pea protein, and studied the DPPH- scavenging rate of the hydrolyzate of the pea protein prepared under different conditions. The hydrolysis conditions were optimized by response surface methodology on the basis of single factor experiments, and the optimized conditions were as follows： hydrolysis temperature 56.5 ℃, time 3.2 h, pH 6.1, alcalase content 3.0% （W/W）, and substrate concentration 3.0% （W/V）. Under the conditions, the DPPH- scavenging rate of the hydrolyzate was up to 47.83%.
出处
《河南工业大学学报（自然科学版）》
CAS
北大核心
2012年第1期6-10,共5页
Journal of Henan University of Technology：Natural Science Edition
