摘要
目的 探讨在我国应用比例法测试结核分支杆菌药物敏感性的可行性以及与以往资料的可比性。方法 用比例法和绝对浓度法测试了 36 0株结核分支杆菌对四种抗结核药物 (INH、SM、EMB、RFP)的敏感性 ,对 19株结果有差别的菌株进行了MIC测试 ,用国产和Sigma公司抗结核药对 30株分支杆菌分别进行了耐药性测定。结果 SM和RFP两种方法检出的耐药率以及与标准结果的一致率均无显著性差异 ,而INH、EMB用比例法测试上述指标均明显高于绝对浓度法 (P <0 0 1)。两种方法试验结果INH、SM、EMB、RFP四药的符合率分别为 :81 1%、92 5 %、83 6 %和 98 6 %。 19株结果有差别菌株的MIC大都分布在两种方法临界药物浓度之间 ,国产和进口药对药敏试验结果的影响无显著性差异。结论 比例法和绝对浓度法结果的差别主要是两种方法的临界药物浓度不同 ,国产药品的纯度和生物活性也可以满足药敏试验的要求。
Objective To analyze the feasibility of proportion method in drug susceptibility testing (DST) of M tuberculosis(MTB) Methods DST was performed to 360 strains of MTB using proportion method and absolute concentration method, 30 of them using both China made and Sigma produced anti TB drugs. MIC was detected in 19 strains whose results were different from standard ones Results In isoniazid (INH) and ethambutol (EMB), the resistance rate and the accordance rate compared with standard results were significantly higher in proportion method than absolute concentration method ( P <0 01) But in streptomycin (SM) and rifampicin (RFP), they did not show any significant difference The accordance rates of INH, SM, EMB and RFP between two methods were 81 1%, 92 5%, 83 6% and 98 6% The DST results using China made and Sigma produced anti TB drugs had no significant difference Conclusions The main reason made proportion method differ from absolute concentration method is critical concentration of drugs China made anti TB drugs are also qualified for DST purpose
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2000年第2期89-92,共4页
Chinese Journal of Tuberculosis and Respiratory Diseases
关键词
结核分枝杆菌
比例法
药物敏感性
Mycobacterium tuberculosis
Proportion method
Susceptibility to drugs
