摘要
利用已构建的包括457个标记位点的大白菜分子遗传图谱,采用多QTL复合作图方法(MQM),通过目测和利用色差计测量两种方法对种皮颜色性状进行了QTL定位和分析。结果表明,共得到种皮颜色的QTLs 9个,其中最重要的QTLs位于A6上,命名为Sc-1,与利用色差计测量法检测到的控制种皮颜色性状L值QTL(ScL-2)和b值(Scb-2)位置完全相同,解释80.4%~100%的表型变异。
183 doubled haploid(DH) lines derived from the cross of two Chinese cabbage( Brassica rapa L. ssp. pekinensis)DH lines, Y177-12 (brown seed)and Y195-93 (yellow seed) , were used to map quantitative trait loci (QTL) related to seedcoat color. Seedcoat color was measured by ocular estimation and colorimeter with CR-400 ( KONICA MINOLTA). Based on the reported genetic map with 457 loci, nine quantitative trait loci (QTLs) were detected for seedcoat color,localizing on linkage groups A4, R6, A7 and R10, respectively. The most significant of the identified QTLs locates on A6,named Sc-1, colocalized with ScL-2 and Scb-2,explaining 80.4% - 100 % of the phenotypic variance.
出处
《华北农学报》
CSCD
北大核心
2012年第1期74-78,共5页
Acta Agriculturae Boreali-Sinica
基金
国家科技支撑计划(2009BADB8B03)
国家大宗蔬菜产业技术体系豫北综合试验站(CAR-25-27)
河南省科技创新杰出人才计划(094200510016)
关键词
大白菜
种皮颜色
QTL定位
Chinese cabbage
Seedcoat color
QTL mapping