摘要
比较研究不同富营养化浅水湖泊中蓝藻种群结构和多样性,对于认识蓝藻水华的区域分布特征具有重要的生态和环境意义。于2009年7月在富营养化严重的滇池、巢湖和太湖各选取4个采样点,采用16S～23S rRNA基因间隔区(Internal transcribedspacer,ITS)序列作为生物标记,通过末端限制性片段长度多态性技术(Terminal restriction fragment length polymorphism,T-RFLP)分析蓝藻种群结构和多样性。实验共得到36个不同的末端限制性酶切片段(Terminal restriction fragment,T-RF),反映3个湖泊中蓝藻种群具有丰富的基因多样性。T-RFLP聚类分析结果表明,在所研究的湖泊中蓝藻种群结构存在空间差异性,其中滇池和巢湖间蓝藻种群结构更为相似。环境因子与蓝藻种群的多元统计分析表明,正磷酸盐(PO4-P)和pH与附着细菌样品蓝藻种群结构动态变化具有显著的相关性(P<0.05),电导率(Conductivity)和总有机碳(TOC)与浮游细菌样品蓝藻种群结构动态变化具有显著的相关性(P<0.05)。因此,不同湖泊蓝藻水华的基因型和驱动因子存在差异。
Cyanobacterial blooming has become of the most challenging environmental issue in shallow lakes in China. Comparative studies on the community composition and diversity of cyanobacteria in shallow lakes of different trophic status and geographic regions will facilitate understanding of the mechanisms of cyanobacterial blooming. In this work, we chosed three eutrophic lakes in different geographic locations inside China for the comparative studies, which include Dianchi, Chaohu and Taihu. We performed field investigations in July of 2009 and applied the terminal restriction fragment length polymorphism（TRFLP） to analyze the cyanobacterial community composition and diversity by using the16S^23S rRNA internal transcribed spacer（ITS） sequences as biomarker. In total, thirtysix terminal restriction fragments （TRFs ） were obtained, indicating relatively high genetic diversity of Cyanobacteria in these lakes. Cluster analysis of TRFLP patterns indicated spatial differences in the cyanobacterial community composition in the three investigated lakes. In general, Lake Dianchi and Chaohu shared similar community composition. Multivariate statistical analysis showed that orthophosphate（PO4-P） and pH were related to（P〈0.05 ） dynamics of cyanobacterial community composition in particleassociated bacterial samples, while conductivity and total organic carbon （TOC） were related（P〈0.05 ） with variations of cyanobacterial community composition in freeliving bacterial samples. Our study indicated that the composition and dynamics of cyanobacterial community and their driving mechanisms may be different in lakes of different trophic status and geographic locations.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2012年第3期570-579,共10页
Journal of Agro-Environment Science
基金
国家自然科学基金"水生高等植物对湖泊浮游细菌种群结构的影响及其机制"(31000241)
中科院南京地理与湖泊研究所领域前沿项目"附生氨氧化原核生物对沉水植物叶面微环境的适应机制"(09SL021001)
