摘要
目的:观察脂多糖(lipopolysaccharides,LPS)反复刺激细胞,诱导产生的内毒素耐受对人牙龈上皮细胞(human gingival epithelial cells,HGECs)分泌细胞因子IL-1β、IL-6和IL-8的影响。方法:采用1mg/L牙龈卟啉单胞菌(Porphyromonas gingivalis,P.gingivalis)LPS或1mg/L大肠杆菌(Escherichia coli,E.coli)LPS刺激HGECs 24h,洗涤细胞后,分别采用相同的LPS再次刺激24h,构建内毒素耐受模型。采用ELISA技术检测细胞条件培养液中IL-1β、IL-6和IL-8分泌水平的变化。结果:P.gingivalis LPS或E.coli LPS刺激HGECs 24h后,3种细胞因子的分泌水平均较刺激前明显增高(P<0.05)。2种LPS重复刺激,诱导细胞耐受后,IL-6和IL-8的分泌水平较第1次刺激后明显降低(P<0.05),但P.gingivalis LPS重复刺激后,IL-1β的分泌水平与第1次刺激后无明显差别。结论:内毒素耐受能抑制HGECs分泌细胞因子IL-6和IL-8,进而可能影响牙周组织的炎症和免疫反应。
Objective： To observe the effects of endotoxin tolerance on the production of IL- 1β, IL-6 and IL-8 in human gingival epithelial cells （HGECs）. Methods： HGECs were stimulated with 1μg/ml Porphyromonas gingivalis （P. gingivalis） lipopolysaccharides （LPS） or lμg/ml （E. coil） LPS for 24h. Then, the cells were washed and stimulated with the same LPS for additional 24h. The levels of IL-1β, IL-6 and IL-8 in supernatants were detected by ELISA. Results： The amounts of IL-1β, IL-6 and IL-8 secreted by HGECs which were stimulated with P. gingivalis LPS or E. coli LPS were increased significantly after 24h （P〈0.05）. After the same stimulations for another 24h, IL-6 and IL-8 production were decreased significantly compared with those secreted by HGECs stimulated with the same LPS only once （P〈0.05）. However, P. gingivalis LPS re-stimulation could not decrease the levels of IL-1β compared with those secreted by the cells stimulated only once. Conclusion： Secretion of IL-6 and IL-8 could be suppressed by endotoxin tolerance, which might have an effect on periodontal inflammation.
出处
《口腔医学研究》
CAS
CSCD
2012年第3期223-226,共4页
Journal of Oral Science Research
基金
国家自然科学基金(编号:81000444)
江苏省自然科学基金(编号:BK2008363)
江苏省高校优秀学科建设工程资助项目
