摘要
目的探讨肠道病毒7l型(EV 71)检测方法和样本收集对检测结果的影响。方法采集疑拟患者鼻咽拭子和大便样本,应用实时荧光定量PCR方法检测。结果结果EV71型肠道病毒大便检出率为46.2%,鼻咽拭子为5.61%,大便的检出率明显高于鼻咽拭子;差异有统计学意义(χ2=61.51>χ20.01,1,P<0.01)。结论对HFMD的诊断建议首选采集粪便标本,应用实时荧光定量PCR方法进行检测,可减少漏诊率,提高灵敏度。
OBJECTIVE To investigate the effects of different detection methods and samples on the results of enterovirus 71（EV71） detection.METHODS The stool and the nasopharyngeal swab samples were detected by real-time fluorescence quantitative polymerase chain reaction（RT·PCR）.RESULTS The positive rate of hand-foot-mouth disease（HFMD） caused by EV71 was 46.2% in the stools,and 5.61% in the nasopharyngeal swab.The determined positive rate in the stools was higher that in the nasopharyngeal swab,the difference statistically significant（χ2=61.51χ20.01,1,P0.01）.CONCLUSION To diagnose HFMD,it is recommended that excrement specimen should be preferred for EV71 detection by real-time fluorescence quantitative PCR,which may reduce the rate of missed diagnosis and raise the detection sensitivity.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2012年第5期1092-1094,共3页
Chinese Journal of Nosocomiology
关键词
手足口病
肠道病毒71型
实时荧光定量PCR
Hand-foot-mouth disease
Enterovirus 7l
Real-time fluorescence quantitative
