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游离NgR基因修饰的骨髓基质细胞移植对脊髓损伤后轴突再生的影响 被引量：1

Effect of free NgR-modified bone marrow stromal cell transplantation on axon regeneration after spinal cord injury
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摘要 目的观察游离NgR基因修饰的骨髓基质细胞（BMSCs）移植对大鼠脊髓轴突再生及神经功能恢复的影响。方法将编码游离NgR蛋白的基因克隆、转染至第3代BMSCs，通过荧光显微镜观察游离NgR蛋白在BMSCs中的表达。另同时制备sD大鼠胸髓挫伤模型，并将其随机分为对照组及实验组，于脊髓损伤1周后分别移植普通BMSCs和游离NgR基因修饰的BMSCs。于细胞移植第1周时采用免疫组化技术检测游离NgR蛋白在损伤区的表达，于细胞移植第4周、第6周时在脊髓损伤区取材，采用髓鞘辣根过氧化物酶逆行染色观察轴突再生情况，并选用BBB评分评估2组大鼠后肢神经功能恢复情况。结果经慢病毒载体转染后BMSCs胞浆内可见明显NgR荧光表达。细胞移植第1周时实验组大鼠脊髓损伤区呈现NgR免疫组化染色阳性；细胞移植第6周时实验组髓鞘染色清晰，分布均匀呈条索状，内有少量空洞形成；对照组髓鞘排列紊乱，内有大量空洞形成。细胞移植第4周及第6周时，发现实验组大鼠BBB评分均显著优于对照组（P〈0．05）。结论通过向脊髓损伤大鼠移植经游离NgR基因修饰的BMSCs，能够通过多种机制促进受损脊髓轴突再生和神经功能恢复。 Objective To evaluate the effect of free NgR-modified bone marrow stromal cell （BMSC） transplantation on axon regeneration in rats after spinal cord injury. Methods Genes encoding free NgR protein were cloned and transduced into BMSCs at passage 3 using a lentivirus vector. Indirect immunofluoreseence was used to detect the expression of free NgR protein, Meanwhile a spinal cord contusion model was established in 36 adult Sprague-Dawley rats at the T10 segment, The rats were then divided randomly into an experimental group and a control group. NgR ＋ BMSCs were transplanted into the injured site 1 week post-trauma in the experimental group. BMSCs were also transplanted at the same time into the control group. Expression of free NgR at the injury site was detected by immunohistochemical staining at 1 week post-transplantation. The functional recovery of both groups was evaluated at 4 and 6 weeks post-transplantation. Longitudinal sections of the spinal cord were studied for axon regeneration u- sing horseradish peroxidase staining. Results Expression of free NgR was found in the cell plasma of BMSCs by indirect immunofluorescence post-transfeetion. Positive immunohistochemical staining for NgR was found at the transplant site in the experimental group 1 week post-transplantation. Better axon plasticity could be observed in the experimental group. The Basso-Beattie-Bresnahan scoring of the experimental group was significantly higher than that of the controls at both observation times. Conclusions Free NgR-modified BMSCs can prompt injured axons to regenerate and thus to promote the recovery of neurological function. This might provide a new strategy to treat spinal cord injury.
作者 潘丽华 马永刚 李亚明 刘世清
机构地区 武汉大学人民医院骨科
出处 《中华物理医学与康复杂志》 CAS CSCD 北大核心 2012年第3期193-196,共4页 Chinese Journal of Physical Medicine and Rehabilitation
关键词 NgR蛋白 骨髓基质细胞 轴突再生 脊髓损伤 康复 NgR protein Bone marrow stromal cells Axon regeneration Spinal cord injury Rehabilitation
分类号 R651.2 [医药卫生—外科学]
  • 相关文献

参考文献13

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二级参考文献20

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共引文献7

同被引文献34

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中华物理医学与康复杂志
2012年 第3期

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