摘要
以北京市延庆县绿富隆有机肥蔬菜研究基地长期定位肥料试验地为试验平台,利用湿筛法获得不同粒级的团聚体,通过16SrDNA-PCR-DGGE技术进行测序分析,研究不同施肥条件下微生物群落对棕壤土团聚体和碳分布的影响。结果表明,有机肥(OF)处理的0.25～2 mm水稳性团聚体增加,增加幅度为109.0%;0.053～0.25 mm和小于0.053 mm粒级团聚体的含量均下降,与CK(不施肥)相比分别下降了31.9%和142.1%。OF处理对土壤各粒级团聚体中碳含量均有显著提高,与CK相比,提高15.2%～46.9%,其中大于2 mm团聚体中碳含量提高了46.9%。棕壤碳含量与大于2 mm粒级团聚体含量呈正相关;与0.25～2 mm粒级团聚体呈极显著正相关;与0.053～0.25 mm粒级团聚体含量呈极显著负相关;与小于0.053 mm粒级团聚体呈显著负相关。
Various particle-size fractions of soil aggregates were obtained with the wet-screening method from the soil of a long-term stationary fertilization experimental field of Beijing Yanqing Lvfulong organic vegetables farms to analyze the effect of microbial communities on carbon distribution in brown soil aggregates under different fertilizations with sequence analysis by 16SrDNA-PCR-DGGE technology. Results showed that with different fertilization treatments, treatments OF （organic fertilizer） increased 0.25 -2 mm water stable aggregates by 109.0% ; compared to CK （unfertilized） , 0. 053 - 0.25 mm and 0- 0.053 mm particle aggregates decreased by 31.9% and 142. 1%, respectively. Treatments OF significantly increased carbon content in aggregates of all particle sizes by 15.2% -46.9% compared to CK, and in the 〉 2 mm aggregates by 46.9%. The content of brown soil carbon was positively related to the content of the 〉 2 mm aggregates; significantly positive related to the content of the 0.25 - 2 mm aggregates, but significantly negative related to the content of the 0. 053 - 0.25 mm aggregates ; negative related to the content of the 0 - 0. 053 mm aggregates.
出处
《农业机械学报》
EI
CAS
CSCD
北大核心
2012年第3期57-61,82,共6页
Transactions of the Chinese Society for Agricultural Machinery
基金
北京市教育委员会科技成果转化与产业项目
北京市属高等学校人才强教计划资助项目(PHR201007140)
关键词
肥料
棕壤
团聚体
群落演替
碳
Fertilizer, Brown soil, Aggregate, Microbial communities, Carbon
