摘要
目的确定重组基底膜提取物（BME）作为细胞外基质促进体外胰岛存活的分子通路。方法分离提纯小鼠胰岛并植入BME凝胶体外培养。蛋白印迹法检测Caspase-3，α1、α5整合素，胰岛功能相关蛋白PDX-1，信号传导相关蛋白Akt、FAK、Erk等的表达。结果研究显示BME可预防胰岛凋亡的发生。和未处理胰岛相比，胰岛植入BME后的Caspase-3表达水平下降和磷酸化Akt表达增加。此外，α3整合素的表达、FAK蛋白水平、磷酸化FAK的表达也显著提高。PDX-1和磷酸化Erk在培养48h时间点的表达显著增加，显示了BME对体外胰岛保存起到了正性调节的作用。结论胰岛体外BME包埋培养可以上调α3整合素及其下游信号转导，以提高其生存能力和胰岛细胞的功能。
Objective To determine the molecular pathway of reconstituted basement membrane extract （BME） embedment in the context of promoting islet cell survival. Methods Mouse islet cells were isolated and embedded in BME for in vitro culture. Caspase-3, integrin-α1 and 5, PDX-1, Akt, FAK and phospho Erk were detected using Western blot. Results Islet cells embedded with BME were partially protected from apoptosis indicated by a lower caspase-3 level and an increased phospho Akt activity compared with untreated control. In addition, an increase of α3-integrin, FAN protein level and FAK activity were observed as well. Furthermore, the expression of PDX-1 and phospho Erk at the 48 h mark were preserved, suggesting the positive effect of BME to islet activity. Conclusion These results indicate that the embedment of BME construction can up-regulate α3-integrin and its signal transduction, which may improve viability and function of islet cells.
出处
《中华肝胆外科杂志》
CAS
CSCD
北大核心
2012年第3期220-225,共6页
Chinese Journal of Hepatobiliary Surgery
基金
基金项目：国家人事部优秀类回国启动基金（BJ2009-44）
北京市自然科学基金（5102039）
关键词
胰岛
α3整合素
细胞外基质
Pancreas islet
α3-integrin
Extracellular matrix
