摘要
为了研究巴氏蘑菇Agaricus blazei多糖对铅中毒大鼠脾脏细胞因子mRNA表达的影响,进而探索巴氏蘑菇多糖对铅中毒大鼠的免疫调节作用。选用45日龄SD大鼠,随机分为6组,每组8只,雌雄各半。分别为正常对照组、多糖组、铅中毒模型组、铅+50mg/kg·d多糖试验组、铅+100mg/kg·d多糖试验组、铅+200mg/kg·d多糖试验组。铅中毒模型组、铅+多糖试验组分别给予含0.2%醋酸铅饮水,自由饮用。饲养60d后,采集脾脏,荧光定量RT-PCR对其细胞因子IL-1β、IL-2、IL-6、TNF-α、IFN-γmRNA的相对表达量进行分析。结果表明,与正常对照组相比,IL-1β、IL-2、IL-6、TNF-α、IFN-γmRNA的表达量,多糖组升高,差异显著(P<0.05或P<0.01);铅中毒模型组下降差异极显著(P<0.01);试验组多糖浓度越高,表达量越高,铅+200mg/kg·d多糖组升高差异极显著(P<0.01)。与铅中毒模型组相比,试验组多糖浓度越高,表达量相对升高越多,铅+200mg/kg·d多糖组升高差异极显著(P<0.01)。说明巴氏蘑菇多糖对铅致大鼠免疫损伤具有保护作用。
To investigate the immunoregulation activity of Agaricus blazei polysaccharides on lead-poisoning rats,the effects of polysaccharides on mRNA expression of cytokines in the spleen of lead-poisoning rats were studied.Forty-eight healthy 45 aged SD rats were randomly divided into six groups（female：male=1：1）,and named as control group,polysaccharide group,lead-poisoning model group,lead＋50mg/kg？d polysaccharide group,lead＋100mg/kg？d polysaccharide group and lead＋200mg/kg？d polysaccharide group respectively.The rats of lead-poisoning model group and lead＋polysaccharide group gave 0.2% lead acetate water for free drink.After treatment of 60 days,the spleens were collected,and the mRNA expressions of cytokines,such as IL-1β,IL-2,IL-6,TNF-α,IFN-γ were determined with fluorescent quantitative real-time polymerase chain reaction（FQRT-PCR）.The results showed that as compared with the control group,the mRNA expression levels of IL-1β,IL-2,IL-6,TNF-α,IFN-γ of polysaccharide group and lead＋200mg/kg？d polysaccharide group significantly increased at 0.05 or 0.01 level,and decreased significantly at 0.01 level in lead-poisoning model group.The mRNA expression levels of the tested cytokines in lead＋200mg/kg？d polysaccharide group increased at 0.01 level as compared with the lead-poisoning model group.The expression levels were incresed with the polysaccharides increased.The result suggested that Agaricus blazei polysaccharides played an improtant role in protecting the lead-induced immune system damage of animal.
出处
《菌物学报》
CAS
CSCD
北大核心
2012年第2期258-266,共9页
Mycosystema
基金
山西省自然科学基金(No.2010011043-1)
山西农业大学博士科研启动经费项目(No.412564)
山西省科技基础条件平台建设项目(No.2010091017)
山西省科技攻关项目(No.20090311047)
关键词
荧光定量
白细胞介素
肿瘤坏死因子Α
Γ干扰素
fluorescent quantitative real-time PCR
interleukin
tumor necrosis factor-α
interferon-γ
