摘要
目的:研究明党参根皮中异欧前胡素、欧前胡素、花椒毒酚、珊瑚菜内酯、5-羟基-8-甲氧基补骨脂素5种呋喃香豆素类成分的体外活性。方法:选取人肝癌细胞株SMMC-7721,HepG2,人肺癌细胞株A-549,人胃癌细胞株MKN-45,人宫颈癌细胞株Hela,人乳腺癌细胞株MCF-7,MDA-MB-2317种肿瘤细胞。以5×104个/mL的细胞密度接种于96孔板,给予不同浓度的药物后培养72 h,采用MTT法观察5种成分对各细胞活性的影响。结果:5种呋喃香豆素对7种肿瘤细胞均表现出不同程度的增殖抑制作用,IC50在0.30～17.23 mg.L-1之间,其中异欧前胡素效果最显著,IC50从0.39 mg.L-1到4.11 mg.L-1。结论:明党参根皮中5种呋喃香豆素类成分具有明显的抗肿瘤活性。
Objective： To study the in vitro activity of five furanocoumarins from the root bark of Changium smyrnioides Wolff. isoimperatorin, imperatorin, xanthotoxol, phellopterin and 5-hydroxyl-g-methoxy- psoralen. Method： The antitumor effect against human liver cancer （SMMC-7721 and HepG2）, human lung cancer （A549）, human gastric cancer （MKN45）, human uterine cervix cancer （Hela） and human breast cancer（ MCF-7 and seven kinds MDA-MB-231 ） was observed by MTT of tumors. IC50 was from 0. 30 mg . L Result： Five furanocoumarins had different inhibition on to 17.23 mg. L-1, especially isoimperatorin was most significantly effective. IC50 was from 0. 39 mg . L-1 to 4. 11 mg. L-1. Conclusion： These five furanocoumarins have obvious antitumor effect.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第6期203-205,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江苏高校优势学科建设工程项目
高等学校博士学科点专项科研基金(200803150009)
江苏省自然科学基金项目(BK2003107)
关键词
明党参根皮
呋喃香豆素
肿瘤细胞
抑制作用
root bark of Changium smyrnioides
furanocoumarin
tumor
inhibition
