摘要
为了解石棉及电焊烟尘相关肺癌的K-ras基因突变情况,并与非职业肺癌的K-ras基因突变特点比较,进一步探讨石棉及电焊烟尘的致癌机理。收集8例非职业肺癌组织、9例石棉相关肺癌组织及4例电焊烟尘相关肺癌组织,提取基因组DNA,经巢式聚合酶链反应(PCR)特异性扩增k-ras基因片段后,进行限制性片段长度多态性分析(RFLP)、单链构象多态性分析(SSCP)及DNA 直接测序分析。K-ras 基因突变百分比在非职业肺癌组为25% ,石棉相关肺癌组为44.4% ,电焊烟尘相关肺癌组为25% ;突变位点方面,非职业肺癌以12密码子为突变热点,石棉相关肺癌则相对集中在12及15密码子,电焊烟尘相关肺癌组则发现在13密码子有突变;碱基替换形式,非职业肺癌以G→T颠换为主,石棉相关肺癌及电焊烟尘相关肺癌以G→A转换为主。结果提示,石棉相关肺癌与非职业肺癌及矽尘相关肺癌有着不同的K-ras基因突变规律,提示三者的发病机理可能不同。由于样本量限制。
To investigate the K ras gene mutations in asbestos and welding fumes related human lung cancer (hLC), comparing with the mutational spectrum of K ras gene in non occupational hLC, 8 cases of non occupational hLC, 9cases of asbestos related hLC and 4 cases of welding fumes related hLC were collected. The techniques of genomic DNA extraction, nested polymerase chain reaction(PCR), polymerase chain reaction restriction fragment length polymorphism(PCR RFLP),polymerase chain reaction single strand conformation polymorphism (PCR SSCP) and DNA direct sequencing were employed. The results:showed the mutation frequencies of K ras gene were 25% in non occupational hLC and welding fumes related hLC, whereas 44 4% in asbestos related hLC. Mutations mainly occurred in codons 12 and 15. A mutation of codon 13 was detected in welding fumes related hLC. The type of base substitution was principally G→T transversion in non occupational hLC, whereas maily G→A transition in asbestos and welding fumes related hLC. The results suggested there were obvious differences of K ras gene mutations among non occupational hLC, asbestos and silica related hLC. Therefore, different mechanisms of caricinogenesis were implicated among them. However, no speific spectrum of K ras gene mutation can be derived from welding fumes related hLC because of the limitataion of samples.
出处
《卫生研究》
CAS
CSCD
北大核心
2000年第1期7-9,共3页
Journal of Hygiene Research
基金
国家自然科学基金!项目(No.39670629)
