摘要
应用小鼠全胚胎培养技术 ,通过妊娠第 8天分别腹腔注射 5 ,10 ,15和 2 0mg·kg-1环磷酰胺 (cyclophosphamide,CP) ,研究了该药对器官原基形成期胚胎的致畸作用 ,并对其致畸机理作了初步探索。给药 4h后取胚胎进行培养 ,于第 10 5天收获胚胎 ,测量其卵黄囊直径、头长及颅臀长并记录其大体形态的变化。实验结果表明 ,15mg·kg-1剂量组引起尾畸形最为显著 ;2 0mg·kg-1剂量组生长迟缓表现较为明显。电镜观察所显示的细胞凋亡及死亡的形态学变化提示环磷酸胺致畸作用可能与其诱导的细胞凋亡和 /或死亡有关。
Using the mouse whole embryo culture technique,the teratogenicity of CP(5,10,15,20 mg·kg -1 )was investigated via intraperitoneal administration to Kun Ming mice on day 8 of gestation,and the mechanism of its teratogenicity was also studied.Embryos were explanted and cultured 4 hours later.On day 10.5,culture was terminated and embryos were examined for yolk sac diameter,head length and crown rump length and teratogenic effects of CP.Significant tail defect was found in CP(15 mg·kg -1 )group and CP induced growth retardation was greatest in CP(20 mg·kg -1 )group.Further results from transmission electron microscopy demonstrated some typical changes of apoptosis and cell death.These findings suggested that CP induced apoptosis and/or cell death might be involved in its teratogenesis.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2000年第2期113-114,共2页
Chinese Journal of Public Health
关键词
环磷酰胺
致畸
全胚胎培养
细胞凋亡
抗癌药
Cyclophosphamide Teratogenicity Whole embryo culture Apoptosis/cell death
