摘要
目的探讨穴位针刺对急性低氧所致大鼠肺动脉压(PAP)升高与神经细胞损伤的干预作用。方法将60只SD大鼠随机平均分为正常对照组(N)、低氧组(H)、针刺+低氧组(AH)。将各组动物分别用25%乌拉坦(125 mg/100 g)腹腔麻醉,H组和AH组(放入低氧舱之前先针刺30 min)动物置于低氧舱后再吸入15%的O2,3组均测量大鼠PAP,30 min后处死。3组均测定脑含水量和大脑腺苷A1受体表达水平,并对不同组大鼠神经细胞进行形态学观察。结果 AH组PAP明显低于H组(P<0.01),但与N组相比差异无统计学意义;海马神经元形态学显示,H组神经元有明显的胞质空染、核固缩,AH组与N组神经元无明显的胞质空染、核固缩。结论穴位针刺可预防大鼠急性低氧引起的PAP升高和海马神经元损伤。
Objective To study the relationship between pulmonary artery pressure （PAP） and neuron injury of hippocampi in rats exposed acute hypoxia. Methods 60 Wistar rats were randomly divided into normal control group, hypoxic group and Acupuncture group, and were hocused by anesthesin, then put hypoxie group and Acupuncture group rats （were aeupunctured 30 minutes first） into hypoxia cabin for 30 minutes, normal group rats were exposure to normal air. Mean PAP of all rats were measured. Results The mean PAP of acupuncture group was lower than that in hypoxic group （ P 〈 0.01 ） , mean PAP was no different between Acupuncture group and normal control group. There were obvious the cytoplasm empty dye, and nuclear condensation in hippocampal neurons of rats in the group H, but not occurred in the group AH, and group N. Conclusion Acupuncture can prevent PAP from increasing and neuron injury of hippocampi in rat.
出处
《山东医药》
CAS
2012年第8期49-51,F0003,共4页
Shandong Medical Journal
基金
国家自然科学基金资助项目(30960522)
关键词
针刺
低氧
肺动脉压
神经元损伤
acupuncture
hypoxia
pulmonary artery pressure
neuron injury
