摘要
以辣椒枣(Ziziphus jujuba Mill.)组培苗叶片为试材,研究了不同培养方式、接种方式以及不同生长调节剂对离体叶片诱导不定芽再生的影响。结果表明,适当的暗处理有利于辣椒枣叶片愈伤组织的形成,并能促进愈伤组织尽快形成芽点,有利于缩短叶片再生培养时间;叶片反放便于愈伤组织及芽点的观察,褐化率低,最终的不定芽再生数高于叶片正放,是辣椒枣叶片再生培养的最佳放置方式。正交试验结果表明,基本培养基,TDZ,IBA和糖4个因子对辣椒枣叶片再生影响大小排序为:基本培养基>糖>TDZ>IBA;1/2 MS(大)为最优培养基,WPM不适合作为辣椒枣的叶片再生培养基;辣椒枣组培苗叶片再生的最优培养基为1/2 MS(大)+TDZ 0.3 mg/L+IBA 0.1 mg/L+糖20 g/L,平均每叶片再生不定芽能达到5.4个。
In the paper,the effect of different culture methods,inoculation methods,growth regulator on Lajiaozao Chinese jujube test-tube seedling leaf regeneration plant was studied.The results showed that： appropriate dark treatment was helpful for the leaf callus formation,and could promote callus to bud as soon as possible to shorten the training time of leaf regeneration.Putting leaf face down was facilitate to callus and bud observation,and it had lower browning rate,higher buds regeneration numbers than putting leaf face up.So it＇s an optimal inoculation method.The orthogonal test results showed： order of effect on Lajiaozao Chinese jujube leaf regeneration performances from big to small was basic culture mediumsugarTDZIBA.1/2 MS was the optimal medium;WPM was not suitable for Lajiaozao Chinese jujube leaf regeneration.In one word,the optimal medium for Lajiaozao Chinese jujube leaf regeneration was： 1/2 MS＋TDZ 0.3 mg/L＋IBA 0.1 mg/L＋20 g/L sugar.
出处
《山西农业科学》
2012年第3期193-196,216,共5页
Journal of Shanxi Agricultural Sciences
基金
山西省回国留学人员科技项目(2009-83)
关键词
辣椒枣
叶片培养
再生植株
最优培养基
不定芽
Ziziphus jujuba Mill.
leaf culture
regeneration plant
the optimum culture medium
adventitious bud
