摘要
目的研究胃癌组织中半胱氨酸蛋白酶-8（Gaspase-8）的表达特点及其与临床病理学特征的关系。方法采用逆转录聚合酶链反应法及免疫组化法检测54例胃癌及其癌周组织中Caspase．8mRNA及蛋白的表达。结果胃癌组织中Caspase-8mRNA表达明显低于癌周组织（0．154±0．065vs0．3944-0．107，t=14．04，P〈0．01），与淋巴结转移、肿瘤分期、分化程度有关（P〈0．01）；胃癌组织中Caspase-8蛋白的阳性率也明显低于癌周组织（31．48％vs85．19％，X^2=32．04，P〈0．01），与肿瘤分期、分化程度有关（P〈0．05）。结论胃癌组织中Caspase-8表达下调，可能与胃癌发生发展密切相关。
Objective To investigate the expression characteristics of Caspase-8 in gastric carcino- ma tissues and its correlation with clinical pathological features. Methods The mRNA and protein of Caspase-8 in gastric carcinoma and its surrounding tissues were detected by reverse transcription polymerase chain reaction （RT-PCR） and immunohistochemistry, respectively. Results The Caspase-8 mRNA in gas- trie carcinoma tissues was obviously lower than that in its surrounding ones （0. 154 ± 0. 065 vs 0. 394 ±0. 107, t = 14. 04, P 〈 0.01 ）, which was correlated with lymph node metastasis, tumor stage, differentia- tion grade （ P 〈0. 01 ）. The Caspase-8 protein in gastric carcinoma tissues was also significantly lower than that in its surrounding ones （31.48% vs 85.19% , X^2 = 32.04, P 〈 0. 01 ）, which was correlated with tumor stage, differentiation grade （ P 〈 0. 05）. Conclusioas Caspase-8 is low-expressed in gastric carci- noma, which may play an important role in the occurrence and progression of gastric carcinoma.
出处
《中国医师杂志》
CAS
2012年第2期166-169,共4页
Journal of Chinese Physician
