摘要
目的:探讨Shh(sonic hedgehog)在大肠腺瘤及大肠癌中的表达及其临床意义。方法:应用免疫组织化学方法检测48例大肠腺瘤及50例大肠癌中Shh蛋白的表达,并与临床病理因素进行相关性分析。结果:Shh蛋白的阳性信号分布在大肠腺瘤和大肠癌细胞质内,在大肠腺瘤和大肠癌中的阳性表达率分别为64.6%(31/48)和58.0%(29/50),明显高于正常组织的20.0%(5/25)(P<0.01,P<0.05);在大肠腺瘤中,Shh的染色评分随腺瘤组织学分级的增加而呈显著性升高(P<0.05)。Shh在早期大肠癌的阳性表达率和染色评分都高于进展期大肠癌,差异均有统计学意义(P<0.01和P<0.0001)。Shh在大肠腺瘤和大肠癌中的表达与性别、年龄、肿瘤部位和大小及组织学类型无关(P>0.05)。结论:Shh信号通路的异常激活在大肠癌发生过程中发挥一定的作用,Shh可作为大肠癌早期诊断的生物学指标及潜在的治疗靶点。
Objective ：To explore the expressions and significance of Shh （sonic hedgehog）in colorectal adenoma and colorectal carcinoma （CRC）. Methods：The expressions of Shh in 48 cases with colorectal adenomas and 50 cases with CRCs were detected and evaluated by immunohistochemistry, and its correlation with clinicopathological factors was statis- tically analyzed to investigate. Results ： Shh protein was detected in the cytoplasm of colorectal adenoma and CRC cells, the positive rate of Shh was in colorectal adenoma and CRC were 64.6% （31/48） and 58.0% （29/50）respectively ,which were significantly higher than that in normal tissue 20.0% （5/25） （P 〈 0.01, P 〈 O. 05 ） ;For staining score evaluation, the expression of Shh in colorectal adenoma were significantly stronger with the increase in histological grade （ P 〈 0.05 ）. Both Shh expressions and staining score evaluation in initial CRCs were significantly higher than that in advanced ones（ P 〈0. 0I, P 〈0.0001 ）. The expression of Shh in the colorectal adenoma and CRC had no correlation with Sex ,age ,tumor position and size,and pathologic type （ P 〉 0.05 ）. Conclusion：The aberrant activation of Shh signaling pathway plays some roles in progression of CRC. Shh may be indicators for early diagnoses of CRC and potential therapeutic targets.
出处
《中华中医药学刊》
CAS
2012年第3期619-621,I0009,共4页
Chinese Archives of Traditional Chinese Medicine
基金
温州市科技局科技资助项目(Y20100236)
关键词
SHH
大肠腺瘤
大肠癌
免疫组织化学
sonic hedgehog
calorectal adenoma
colorectal carcinoma
immunohistochemistry
