摘要
目的建立七厘散中儿茶素和表儿茶素的含量测定方法。方法 HPLC法,采用Prevail Select C18(150 mm×4.6mm,5μm)色谱柱,柱温:35℃;以乙腈-水-磷酸(100∶900∶1)为流动相;流速:1 ml/min;检测波长:278 nm。结果儿茶素、表儿茶素线性浓度范围分别为30.08～150.4μg/ml,r=0.9999;12.24～61.20μg/ml,r=0.9999;回收率分别为99.1%和97.4%,RSD分别为1.1%和1.2%。结论本方法操作简便,测定结果准确可靠,可用于七厘散中儿茶素和表儿茶素的含量测定。
Objective To establish a method for determining catechin and epicatechin in Qili San.Methods HPLC was used for the determination.The chromatographic column was Prevail Select C18（150 mm ×4.6 mm,5 μm）and the column temperature was 35℃.The mobile phase consisted of acetonitrile-water-phosphoric acid（100∶ 900∶ 1）.The flow rate was 1 ml/min and monitored at 278 nm.Results The linear range of catechin was 30.08-150.4μg/ml,r=0.9999.The average recovery of catechin was 99.1% and RSD was 1.1%.The linear range of epicatechin was 12.24-61.20μg/ml,r=0.9999.The average recovery of epicatechin was 97.4% and RSD was 1.2%.Conclusion This method is simple,time-saving and accurate.It can be used for routine analysis of catechin and epicatechin in Qili San.
出处
《解放军药学学报》
CAS
2012年第1期72-73,76,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
