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幽门弯曲菌分离培养及其鉴定技术的研究 被引量:1

STUDY ON CULTURE AND IDENTIFICATION OF CAMPYLOBACTER PYLORI
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摘要 本文报告了利用改良Skirrow血平板和活组织直接涂片镜检对90例上消化道疾病患者的胃活检组织标本进行了幽门弯曲菌检测。涂片镜检阳性率为81.1%,分离培养阳性率为54.4%。该菌仅在微氧环境中生长,氧化酶、触酶、尿素酶均阳性,对萘啶酮酸抵抗。涂片镜检和快速尿素酶试验快速、简便,可做为幽门弯曲菌分类、鉴定的重要方法。 Biopsy specimens gastric mucosa from 90 pati- ents with upper gastrintestinal complaint were stu- died by direct microscopy and culture. The result showed that 81. 1% biops contained spiral bacteria by microscopic examination, and only 54. 4% positive with culture. The spiral bacilli grow only under mi- croaerophilic condition. All isolates wore oxidase, catase. urease positive, resistant to nalidixic acid. Direct smear slid and Rapid urease test are simple, easy to perform. They can be used in any laboratory for both the identification and the classification
出处 《中国公共卫生学报》 1990年第3期159-161,共3页
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