摘要
通过硫酸钠—DEAE纤维素法,胃酶消化法及流基乙醇还原法制备了免抗A群流脑菌Fab’片断。所得到的Fab’片断通过一种异双功能基因交联剂HECCM与HRP连接,成功制备了Fah’-HRP交联物。后者通过凝胶过滤层析鉴定,分子量为86099。该交联物用于ELISA双抗体夹心法检测流脑A群菌标准抗原,敏感度达0.225ng/ml。
Fab' fragments was prepared using sodium su- fate-DEAE cellulose chromatography, digestion w- ith pepsin and reduction with 2-mercaptoenthnol. Then the HRP was cross-linked to Fab' through HECCM to form Fab'-HRP conjugate. The MW of the conjugate was found to be 86,099 with gel filt- ration chromatography. Fab'-HRP conjugate was applied to sandwich ELISA and the Fab'-ELISA was established. The sensitivity of the method to detect APS was 0. 225ng/ml.
