摘要
为了研究棉花SUS3基因编码区的第1个内含子在基因表达调控中的作用,分离该内含子并将其插入到GUS基因编码区中构建载体Susfig::121,将载体Susfig::121通过农杆菌介导转入拟南芥。对转基因拟南芥进行GUS活性分析,发现该内含子对GUS基因在拟南芥中的表达具有负调控作用,并且特异抑制报告基因在花粉中表达。其结果显示,棉花SUS3基因编码区的第1个内含子对基因的表达具有一定的调控作用。
Cotton sucrose synthase 3 （SUS3） plays an important role during the cotton fiber development. Compared to Arabidopsis SUS1/SUS4, the gene structure of cotton SUS3 has a high similarity with that of those two genes. To determine the function of the first intron of cotton SUS3, the intron was isolated and inserted into the GUS coding region to construct vector Susfig：121, and which was transformed into Arabidopsis thaliana mediated by agrobacterium. The results of GUS activity analysis of transgenic Arabidopsis plants indicated that the intron down-regulated GUS gene expression in non-pollen tissues and specifically repressed GUS expression in pollen, which suggested that the first intron played a role in gene expression.
出处
《热带生物学报》
2011年第4期291-296,共6页
Journal of Tropical Biology
基金
国家转基因生物新品种培育重大专项资助项目(2009ZX08005-027B)
