摘要
目的:研究开口箭根茎中甾体成分,寻找新的抗肿瘤活性物质.方法:运用反相常压柱色谱和制备高效液相对其甾体成分进行分离,通过理化性质和波谱分析方法鉴定其结构.结果:从开口箭根茎的乙醇提取物中分离得到6个甾体皂苷及2个甾体皂苷元,其结构分别为1β,2β,3β,4β,5β-五羟基-螺甾-△25(27)-烯-5-O-β-D-吡喃葡萄糖苷(1),1β,3β-二羟基-5β-孕甾-△16(17)-烯-20-酮-3-O-β-D-吡喃葡萄糖苷(2),1β,2β,3β,4β,5β,6β,7α-七羟基-5β-呋甾-△25(27)-烯(3),1β,2β,3β,4β,5β,7α-六羟基-6-氧化-螺甾-△25(27)-烯(4),3-O-β-D-吡喃葡萄糖基-(25S)-1β,3β,5β,26-四羟基-5β-呋甾-△20(22)-烯-26-O-β-D-吡喃葡萄糖苷(5),3-O-β-D-吡喃葡萄糖基-(25S)-1β,3β,5β,22α,26-五羟基-5β-呋甾-26-O-β-D-吡喃葡萄糖苷(6),3-O-β-D-吡喃葡萄糖基-(25S)-1β,3β,26-三羟基-5β-呋甾-26-O-β-D-吡喃葡萄糖苷(7)和3-O-β-D-吡喃葡萄糖基-(25R)-1β,3α,26-三羟基-△5(6)-烯-5β-呋甾-26-O-β-D-吡喃葡萄糖苷(8).结论:化合物1和2为首次从该属植物中获得.
To study the steroidal saponins from the fresh rhizomes of Tupistra chinensis and search for bioactive saponins with potential antitumor activity.The steroidal saponins were isolated by RP-C18 column chromatography and preparative HPLC.The structures of the compounds were dertermined on the basis of the spectroscopic analysis and chemical evidences.Eight spirostanol compounds（1-8） were obtained from the n-butanol fraction of EtOH extract from Tupistra chinensis rhizomes.Their structures were determined on the basis of chemical methods and spectral data.Compounds 1 and 2 were isolated from this plant for the first time.
出处
《三峡大学学报（自然科学版）》
CAS
2012年第1期85-88,共4页
Journal of China Three Gorges University：Natural Sciences
基金
国家自然科学基金项目(30870254
31070313
30670213)
湖北省自然科学基金创新群体项目(2007ABC008)
关键词
开口箭
甾体皂苷
呋甾皂苷
螺甾烯
Tupistra chinensis
steroidal saponin
furostanol saponin
spirostane
