摘要
本文采用薄层聚丙烯酰胺凝胶等电聚焦电泳检测红细胞及血痕酸性磷酸酶表型,并对不同条件下的血痕标本进行检测,发现室温下(15～33℃)保存的110例纱布血痕7周内可全部正确分型,21例磁板血痕9周内均可正确分型;含血量≥5λl 的血痕可被正确检出 EAP 表型;日晒、水洗、发霉等因素可影响血痕 EAP 型的正确检出。同时调查了广东人群的 EAP 表型分布,基因频率为 p^a=0. 2338,p^b=0. 7662,发现 EAP 基因频率分布存在着地区差异。
The 340 blood samples from Guangdone area were detected by ultra thin poly-
acrylamide gel isoelectric focusing for EAP phenotypes.The phenotypes were
20 EAP A,119 EAP BA,201 EAP B.The gene frequencies of EAP were as foll- ows p^(?)0. 2338,p^(?)0. 7662. The 110 bloodstain samples of the cloth kept at room
temperature for 7 weeks could be phenotyped correctly,The 21 bloodstain samp-
les on the porcelain plate kept at room temperature for 9 weeks could be phen-
otyped correctly.When the blood volume of bloodstain was equal to or over
5μl EAP could be phenotyped.6 out of 20 mouldy bloodstains,the EAP BA
phenotype were changed to EAP B.In blind trial,20 bloodstain samples kept at
room temperature for 7 weeds could be phenotped correctly.
出处
《中国法医学杂志》
CSCD
1990年第2期86-89,共4页
Chinese Journal of Forensic Medicine
关键词
血痕
酸性磷酸酶
基因频率
Isoelectric focusing(IEF)
Erythrocyte acid phosphatase(EAP)
Gene frequencies
Bloodstain
