摘要
目的探讨过表达血管内皮生长因子(VEGF)对垂体瘤微血管生成的影响。方法用携带VEGF165基因的腺病毒质粒转染垂体瘤MMQ细胞,并建立稳定的过表达VEGF165的MMQ细胞株。制备鸡胚尿囊膜血管生成模型(CAM),观察过表达VEGF165 MMQ细胞的上清液对CAM血管生成的影响。分别接种过表达VEGF165 MMQ细胞、空载体细胞和正常MMQ细胞于裸鼠皮下并形成皮下移植瘤。CD31免疫荧光法检测过表达VEGF基因对裸鼠皮下MMQ细胞移植瘤微血管密度的影响。结果大部分转染组细胞镜下呈现绿色荧光,RT-PCR和Western blot证实转染携带VEGF165基因的腺病毒质粒(Adv-GFP-VEGF165)的MMQ细胞中目的基因能够有效表达。VEGF过表达能够诱导CAM局部血管生成,并显著增加MMQ细胞裸鼠皮下移植瘤的微血管密度。结论 VEGF在垂体瘤血管生成过程中起重要作用。
Objective To investigate the effect of vascular endothelial growth factor（VEGF） overexpression on angiogenesis in pituitary adenomas.Methods A stable MMQ cell line overexpressing VEGF165 gene was constructed by via recombined adenovirus transfection.The effects of VEGF165 overexpression on the angiogenesis in the chorioallantoic membrane（CAM） and subcutaneous xenograft MMQ cell tumors were recorded.Results Most MMQ cells transfected with Adv-GFP-VEGF165 displayed green immunoflurescence and higher mRNA and protein levels of VEGF.VEGF165 overexpression induced the angiogenesis in chorioallantoic membrane and MMQ pituitary tumor cell xenografts.Conclusion VEGF may play an important role in the angiogenesis of pituitary adenomas.
出处
《安徽医科大学学报》
CAS
北大核心
2012年第3期261-265,共5页
Acta Universitatis Medicinalis Anhui
基金
美国癌症协会IRG基金(编号:RSG091381CNE)
