摘要
目的探讨干扰素-γ(IFN-γ)对RAW264.7巨噬细胞(Macrophage,Mφ)体外抗阿萨希毛孢子菌(Trichosporon asahii,T.asahii)杀伤作用的机制。方法不同浓度(10、100、1000U/ml)的IFN-γ与RAW264.7共同培养,再加入阿萨希毛孢子菌培养后检测菌落形成单位(CFU/ml),计算各生长抑制率。在上述体系上清液中分别检测亚硝酸根离子(NO2-)。将巨噬细胞与不同终浓度的IFN-γ共同孵育后,分别检测超氧阴离子(O2-)的OD值。结果 IFN-γ浓度分别为10、100、1000U/ml时,T.asahii的生长抑制率分别为25.21%、41.95%、55.83%,组间差异具有显著统计学意义(P<0.01);上清液中NO2-的浓度分别为89.16±3.32、108.07±3.81、126.44±3.52,与空白对照组(74.47±1.48)相比差异具有显著性意义(P<0.01);上清液中O2-的OD值分别为0.317±0.006、0.424±0.006、0.518±0.006,与对照组相比差异具有显著性意义(P<0.01)。结论随着IFN-γ浓度的增加,T.asahii生长抑制率增加。IFN-γ通过增强Mφ产生一氧化氮(NO)及超氧阴离子(O2-)来发挥体外抗T.asahii的杀伤作用。
Objective To investigate the effect of IFN-γ on the killing mechanism of RAW264.7 macrophage against Trichosporon asahii（T.asahii） in vitro.Methods The various concentrations（10,100,1000U/ml） of interferon-γ with RAW 264.7 were incubated,and then cultured with T.asahii to assess the killing activity by counting the number of colony forming units per milliliter（CFU/ml） of T.asahii.Then calculate the T.asahii growth inhibition rate,and measure Nitrite ion（NO2-） in the supernatant respectively.Each well added by the above different terminal concentrations of IFN-γ incubated with RAW264.7 to determine the superoxide anion（O2-） respectively in the supernatant.Results With the concentration of 10,100 and 1000U/ml of IFN-γ,the growth inhibition rates of T.asahii were 25.21%,41.95%,55.83% respectively.There was significant difference between each group（P0.01）.In the supernatant the concentrations of Nitrite ion were 89.16±3.32,108.07±3.81,126.44±3.52 respectively.There were significant difference（P0.01） compared to the blank control group（74.47±1.48）.With the concentrations of IFN-γ rising（10,100,1000U/ml respectively）,the OD value of the O2-measured in the supernatant were increasing（0.317±0.006、0.424±0.006,0.518±0.006 respectively）.And the blank control group was 0.222±0.008,there was significant difference between each group（P0.01）.Conclusion With the concentrations of IFN-γ rising,the growth inhibition rates of T.asahii are increasing.IFN-γ plays a role in killing effect of T.asahii in vitro by enhancing macrophage producing nitrogen monoxidum（NO） and superoxide anion（O2-）.
出处
《实用皮肤病学杂志》
2012年第1期5-8,共4页
Journal of Practical Dermatology
基金
国家自然基金(编号:81071304)
