摘要
目的观察补肾活血方对Aβ联合D-半乳糖诱导大鼠老年性痴呆动物模型认知功能的干预作用及其机制。方法脑立体定位仪双侧海马内一次性脑内注射Aβ25-35联合长期颈部皮下注射D-半乳糖制作大鼠AD动物模型,注射24h后灌胃给药,连续4周,以Morris水迷宫实验进行认知行为学检测,ELISA法测定脑组织中SOD、MDA含量。结果补肾活血方组大鼠在安全岛象限的搜索时间和搜索距离较模型组大鼠明显延长。补肾活血方还可降低大鼠脑组织MDA含量,升高SOD活性水平。结论补肾活血方可明显改善Aβ联合D-半乳糖诱导的AD大鼠的学习记忆能力,其作用可能与调节脑内脂质过氧化物反应有关。
Objective To observe the therapeutic effect of Bushen huoxue formula to experimental animal model of Alzheimer＇s disease.Methods established AD animal model with Aβ25-35 encephalic injection once and D-galactose hypodermical injection for 5 weeks,then administrated the rats with drugs for 4 weeks,after that,check the cognitive behavior with Morris mater maze experiment,and survey the contents of SOD and MDA of the brains.Results After administrated the animal with Bushen huoxue formula,the searching time and distance in safety island quadrant were lengthened.The formula also diminished the content of MDA and elevated the activity of SOD.Conclusion Bushen huoxue formula obviously improved the learning and memonic capacity of AD rats,its mechanism may has certain relationship to its regulating lipid peroxidation reaction effect.
出处
《中国医药科学》
2012年第4期32-33,38,共3页
China Medicine And Pharmacy
基金
国家国际科技合作项目(2010DFB33260)
