摘要
以反相悬浮交联法制备磁性壳聚糖/β-环糊精复合微球。用光学显微镜、红外光谱仪、振动样品磁强计(VSM)对微球进行了表征,并研究其对模型蛋白(木瓜蛋白酶)的吸附行为。结果表明,微球为完整的球形,具有良好的磁响应性。模型蛋白在微球上的吸附关系既可用Langmuir方程描述,又可用计量置换吸附Freundlich方程描述,且更符合Freundlich方程描述;聚丙烯酰胺凝胶电泳(SDS-PAGE)结果表明,吸附的模型蛋白能从磁性粒子表面完整地脱附下来。
A series of novel magnetic microspheres were prepared by inverse suspension crosslinking method from chitosan, β-cyclodextrin and Fe304. The obtained magnetic microspheres were characterized by means of optical microscope, IR and VSM. The adsorption behaviour of those microspheres towards papain was investigated. The results show that the microspheres were in a spherical shape with good magnetic response. The adsorption behaviour of the microspheres towards papain was described by both the Langmuir adsorption isotherm and the Freundlich isotherm, which shows that the adsorption behaviour fitted more to the Freundlich isotherm. Sodium dodecyl sulfate- polyacrylamide gel electrophoresis （ SDS - PAGE） shows that the papain could be desorbed from the magnetic microspheres without denaturation.
出处
《精细化工》
EI
CAS
CSCD
北大核心
2012年第3期280-284,共5页
Fine Chemicals
关键词
壳聚糖
Β-环糊精
磁性微球
医药原料
chitosan
β-cyclodextrin
magnetic microspheres
drug materials
