摘要
背景:骨延长的牵张刺激如何转变为骨质再生的生物学信息至今不明确。目的:观察骨延长患者诱导型一氧化氮合酶的动态表达。方法:单侧胫腓骨延长组患者8例,分别于手术前第3天、术后第3,7,8,11,30天、停止延长时、停止延长第3,15,30天、拆除外固定器时、拆除外固定器后30d,检测血清诱导型一氧化氮合酶水平。并设立年龄相匹配的对照组8例。结果与结论:骨延长组牵张操作开始第1天时(术后第8天),血清诱导型一氧化氮合酶即开始升高,停止延长时达高峰,这一时段各个时相骨延长组血清诱导型一氧化氮合酶均显著高于对照组(P<0.01),停止延长3d时的血清诱导型一氧化氮合酶亦高于对照组(P<0.05)。提示诱导型一氧化氮合酶的高表达是骨延长的分子生物学机制之一。
BACKGROUND： It is unknown that how to transfer the impetus of distraction into the message of bone regeneration in limb lengthening. OBJECTIVE： To study the dynamic expression of inducible nitric oxide synthase （iNOS） in bone lengthening patients. METHODS： Totally eight patients whose tibia and fibula are shorten in single lower limb were involved in bone lengthening group. The serum iNOS levels of the patients were measured respectively at 3 days preoperatively, at 3, 7, 8, 11, 13 days postoperatively, stopping bone lengthening, at 3, 15, 30 days after stopping bone lengthening, removing the external lengthening apparatus and at 30 days after removing the external lengthening apparatus. Eight patients with similar age to the bone lengthening group were selected as control. RESULTS AND CONCLUSION： The serum iNOS levels in the bone lengthening group began to increase at first day after the distraction （the eighth day after operation） and the peak values was at the stopping bone lengthening. The expression of iNOS in the bone lengthening group was significantly higher than that of the control group at every time point from the first day after the distraction to the day of stopping bone lengthening （P 0.01）. The serum iNOS levels in the bone lengthening group at 3 days after stopping the bone lengthening was also higher as compared with the control group （P 0.05）. It is indicated that the higher expression of iNOS is one of the molecular biological mechanisms of bone lengthening.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
2012年第4期709-712,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
