摘要
采用砂培方法,在温室内研究了马尾松1年生苗木在不同锰浓度(0.005(对照)、1、5、10和15mmol·L-1)条件下的生理指标响应。结果表明:与对照相比,高锰浓度(≥10mmol·L-1)下马尾松的生物量和根系活力降低。高锰条件下,马尾松针叶中叶绿素a、叶绿素b、类胡萝卜素含量下降,表明锰对马尾松光合机构存在一定影响,同时也是导致生物量降低的原因之一。在高锰胁迫下,丙二醛(MDA)含量以及超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性均发生显著变化且对锰毒起到一定的缓解作用,其中CAT和MDA对高锰胁迫的响应比较灵敏,MDA和2种酶的变化反映了马尾松对高锰胁迫的生理响应。
A greenhouse sand culture experiment was conducted to study the physiological responses of masson pine (Pinus massoniana) seedlings to different concentrations (0.005, 1, 5, 10 and 15 mmol·L-1) of manganese (Mn). As compared with the control (0.005 mmol·L-1 of Mn), high Mn concentrations (10 and 15 mmol·L-1) decreased the seedling’s biomass and root activity and the needle’s chlorophyll a, chlorophyll b, and carotenoid contents, indicating that Mn had certain impacts on the needle photosynthetic framework, and also, was one of the reasons leading to the decrease of seedling biomass. Under the stress of high Mn concentrations, the needle superoxide dismutase (SOD) and catalase (CAT) activities and malondialdehyde (MDA) content changed significantly, and played definite roles in mitigating Mn toxicity. The CAT and MDA were more sensitive to Mn, and the changes in the SOD and CAT activities and MDA content reflected the physiological responses of pine seedlings to high-Mn stress.
出处
《生态学杂志》
CAS
CSCD
北大核心
2012年第3期520-525,共6页
Chinese Journal of Ecology
基金
国家"十一五"科技支撑课题(2006BAD24B0301)
国家科技成果转化课题(2007GB2F200286)
贵州省重大专项课题
贵阳市科技局社会发展研究专项(2009-3-0419)资助
关键词
马尾松
锰浓度
胁迫
生理指标
masson pine
manganese concentration
stress
physiological index.