摘要
利用荧光探针和免疫组织化学方法,研究了乌贼墨对H22癌细胞内Ca2+浓度、核膜Ca2+/Mg2+-ATP酶活性及c-jun表达的影响。结果发现,乌贼墨使H22癌细胞内Ca2+浓度分别降低了69%和79%,核膜Ca2+/Mg2+-ATP酶活性分别降低了21%和37%,c-jun表达明显减少。结果提示乌贼墨可能通过降低细胞内Ca2+浓度,继而影响到核膜上Ca2+依赖性Ca2+/Mg2+-ATP酶活性,减少了Ca2+向细胞核内的转运,使Ca2+对c-jun表达的促进作用减弱,抑制了细胞的分裂增殖。这可能是乌贼墨抑制肿瘤生长的机制之一。
The effect of sepia on the intracellular Ca 2+ concentration,nuclear Ca 2+ /Mg 2+ ATPase activities and the expression of c jun were studied in H 22 cancer cells by using fluorescent probe and immunohistochemical method.The results showed that intracellular Ca 2+ concentration decreased 69% and 79%,nuclear Ca 2+ /Mg 2+ ATPase activities diminished 21% and 37%,c jun expression decreased obviously.The results suggested that sepia probably diminished the intracellular Ca 2+ concentration,affected Ca 2+ dependent nuclear Ca 2+ /Mg 2+ ATPase activities,thus reduced the amount of Ca 2+ transporting into nuclei,so lessened the promoting effect of Ca 2+ on c jun expression,and therefore inhibited cellular differentiation and proliferation.This might be one of the possible anti cancer mechanisms of sepia.
出处
《卫生研究》
CSCD
北大核心
2000年第2期96-98,共3页
Journal of Hygiene Research
基金
山东省卫生厅科学基金资助!( No.90 C0 83 0 )
