摘要
为探讨预热对肿瘤细胞肿瘤坏死因子α(TNF-α)敏感性的影响及其机理,将白血病患者胸腔渗出细胞系(K562细胞)在40℃预热不同时间(0、30、60、90、120、150和180min),使细胞热应激蛋白70(HSP70)高表达;然后将预热60min细胞和未预热细胞分别暴露于50、100、200和400U/ml的TNF-α16小时,用MTT比色法检测细胞活力。保持TNF-α浓度为100U/ml不变,观察预热时间对细胞TNF-α敏感性的影响,用逆转录聚合酶链反应(RT-PCR)测定细胞HSP70的含量。结果:40℃预热后细胞的HSP70含量升高,在预热120min达到最高,以后缓慢下降;预热细胞对相同浓度TNF-α的敏感性明显低于未预热细胞;对于相同浓度的TNF-α,预热120min的细胞对TNF-α敏感性最低。结果提示,预热可以引起细胞HSP70表达增高,降低细胞对TNF-α的敏感性。
In order to study the effects of pre heating on the sensitivity of cells to tumor necrosis factor α(TNF α), the K 562 cells were pre heated at 40℃ for different times to induce heat shock protein 70(HSP70). To observe the effects of TNF α concentration on the sensitivity of pre heated cells, cells were pre heated for 60 min and treated with TNF α (50,100,200 and 400U/ml) for 16 hours, and the cells vitalities were detected by MTT. In order to observe the effect of pre heating time on the sensitivity of cells to TNF α,the pre heated cells were treated with 100U/ml TNF α for 24 hours. The levels of HSP70 were tested by reversed transcription PCR(RT PCR). The results showed that HSP70 could be induced by pre heating, and reached its top level after 120mins, and the sensitivity of non heated cells to TNF α was lower than that of pre heated cells. To 100U/ml TNF α, the sensitivity was lowest after the cells were pre heated for 120 min. The results suggested that pre heated could induce the expression of HSP70 and inhibite the sensitivity of cell to TNF α, the mechanism may be involved with the overexpression of HSP70.
出处
《卫生研究》
CAS
CSCD
北大核心
2000年第2期87-89,共3页
Journal of Hygiene Research
