摘要
为探讨甲基丙烯酸环氧丙酯(GMA)诱导细胞转化的机理,选用划痕染料示踪技术(SLDT)初步研究了GMA对人胚肺成纤维细胞间隙连接通讯(GJIC)的影响。用0.5、2.5和5.0mg/L的GMA给细胞染毒12h,经划痕导入荧光黄染料并测定各组细胞中该荧光染料向其相邻细胞扩散的程度。结果表明,在染毒剂量及时间范围内,GMA对人胚肺成纤维细胞GJIC产生较强的抑制作用,呈良好的剂量-效应关系。其中,2.5和5.0mg/L剂量组细胞的GJIC明显降低。结果提示GMA对细胞间隙通讯的抑制作用可能是其导致细胞恶性转化的重要机制之一。
In order to probe into the mechanism of glycidyl methacrylate (GMA) induced cell tansformation,the effects of GMA on gap junctional intercellular communication (GJIC) in human lung, embryonic fibroblasts(HLEF) were studied by using scrape loading and dye transfer(SLDT) technique.HLEF cells were treated with GMA at concentrations of 0 5,2 5 and 5 0mg/L for 12 hours and Lucifer Yellow was introduced by scrape loading after exposure.The GJIC in exposed cells and that of controls were determined directly as the diffusion extent of fluorescent dye into adjacent cells.The results indicated that GMA could inhibit the GJIC in exposed cells in a dose dependent manner.Significantly decreased GJIC was found in 2 5 and 5 0mg/L dose groups.It was suggested that the inhibition of GJIC might be one of the mechanisms responsible for GMA induced cell transformation.
出处
《卫生研究》
CAS
CSCD
北大核心
2000年第2期73-75,共3页
Journal of Hygiene Research
基金
国家自然科学基金! ( No.3 9840 0 17)
关键词
甲基丙烯酸
环氧丙酯
GMA
GJIC
肺成纤维细胞
癌
glycidyl methacrylate,gap junctional intercellular communication,human lung embryonic fibroblasts
