摘要
采用高效液相色谱-紫外检测方法,结合外标法建立了乙酰甲胺磷原药测定的分析方法。研究了流动相组成等因素对色谱分离效果的影响,得到了最优化的分离测定条件:采用C18柱,以乙腈—水(3∶97,v/v)为流动相,流速1.0mL/min,紫外检测波长为210 nm,乙酰甲胺磷和甲胺磷在3 min内完全分离。将该方法应用于乙酰甲胺磷原药测定上,回收率介于98.9%～100.4%之间,相对标准偏差介于6.3%～7.2%之间,效果较好。
The analysis method for determination of acephate by High Performance Liquid Chromatography （HPLC）, combining with external standard method was established. Several factors effects on chromatography separation were studied, as well as the component of mobile phase. The optimum condition for separation was obtained. The two analytes were completely separated in ACN ： Water （3 ： 97,v/v ） within 3 minutes with flow rate of 1.0 mL/min UV-Vis detection of 210 nm wavelength. The potential method has been used to determine the acephate samples. The result showed that the average spiked recoveries ranged from 98.9% to 100.4%, the relative standard deviation was between 6.3%-7.2%, which had a good result.
出处
《宁夏农林科技》
2011年第12期231-232,共2页
Journal of Ningxia Agriculture and Forestry Science and Technology
