摘要
以栽培紫苏为材料,通过对其离体茎段的组织培养,建立了诱导、分化、增殖、生根等一整套离体快繁体系。研究结果表明,紫苏茎段外植体较好的灭菌方法为:75%酒精30s+0.1%HgCl210min;茎段外植体初代培养的适宜培养基为MS+2mg.L-1 6-BA+0.5mg.L-1 NAA;继代及增殖培养以6-BA 2mg.L-1与NAA0.1mg.L-1配比时效果较好;试管苗生根培养的最适宜培养基是1/2MS基本培养基附加0.2mg.L-1 NAA。
In this paper,with the stems of Perilla frutescens as explants,a set of propagation system in vitro about the tissue culture of induction,differentiation,proliferation,rooting was established.The results showed that the optimal sterilization method for stems of Perilla frutescens was 75 % alcohol 30 s＋0.1 % HgCl2 10 min;the optimal early culture medium was MS＋2 mg·L-1 6-BA＋0.5 mg·L-1 NAA,on which the stem explants could grow vigorously,the bud differentiation amount and induction rate were higher;the optimal proliferation culture medium among all tested media was 2 mg·L-1 6-BA＋0.1 mg·L-1 NAA;the optimal rooting culture medium among all tested ones was 1/2MS basic medium added 0.2 mg·L-1 NAA.
出处
《山西农业大学学报（自然科学版）》
CAS
2011年第6期498-501,共4页
Journal of Shanxi Agricultural University(Natural Science Edition)
基金
国家863项目(2006AA100204)
山西农业大学博士科研启动基金(412568)
关键词
紫苏
组织培养
快速繁殖
茎段
Perilla frutescens
Tissue culture
Rapid Propagation
Stems
