摘要
以成熟文冠果叶片作为外植体,通过器官直接发生途径,对其进行不定芽诱导、不定芽增殖及生根培养,建立了一种简单易行的、可再生的文冠果快速繁殖方法。结果表明,在不定芽诱导阶段,当MS培养基添加2.0mg.L-1BA和0.2mg.L-1 NAA时,不定芽诱导率最高;高浓度的BA显著降低不定芽诱导率;当MS培养基添加2.0mg.L-1 BA和0.1mg.L-1 NAA,每个外植体获得的不定芽数达到最大;当叶片远轴面接触培养基时,不定芽诱导率高于近轴面接触培养基。在增殖培养阶段,当MS培养基添加0.5mg.L-1 BA时,不定芽增殖率、增殖个数及增殖芽长均达到最大;在不同浓度的BA培养基中添加NAA降低了不定芽的增殖效果。在生根培养阶段,1/2MS培养基添加0.5mg.L-1 IBA的生根率最高;IBA的生根效果显著优于NAA或IAA。生根苗在含有土壤、沙子和泥炭的混合基质(1∶1∶1)中移栽成活。
An efficient and reproducible protocol was developed to induce direct organogenesis from leaf explants of Xanthoceras sorbifolia Bunge.,a potential bioenergy tree.The highest frequency of shoot induction was observed on Murashige and Skoog（MS） medium supplemented with 2.0 mg·L-1 6-benzylaminopurine（BA） and 0.2 mg·L-1 naphthaleneacetic acid（NAA） at a rate of 96.7%.Higher concentrations of BA resulted in significant decrease of shoot induction frequency.The greatest number of shoots per explant was obtained on medium containing 2.0 mg·L-1 BA and 0.1 mg·L-1 NAA.When in contact with the medium,the abaxial surface of leaf explants was better than the adaxial surface in shoot induction frequency.The highest shoot multiplication rate,number of proliferated shoots and shoot length were observed on MS medium containing 0.5 mg·L-1 BA.Addition of NAA to the medium with different concentrations of BA decreased the multiplication efficiency.The highest frequency of rooting（51.1%） was obtained on half-strength MS medium（1/2 MS） combined with 0.5 mg·L-1 indole-3-butyric acid（IBA）.The efficiency of rooting on medium supplemented with IBA was significantly higher than that on medium with NAA or indole-3-acetic acid（IAA）.Rooted plantlets were acclimatized in pots containing 1∶1∶1 mixture of soil,sand and peat.
出处
《山西农业大学学报（自然科学版）》
CAS
2011年第6期492-497,共6页
Journal of Shanxi Agricultural University(Natural Science Edition)
基金
山西省林业厅科技创新项目(2010001)
山西省研究生优秀创新项目(20093061)
关键词
文冠果
叶片外植体
器官直接发生
增殖
生根
Xanthoceras sorbifolia
Leaf explant
Organogenesis
Proliferation
Rooting
