摘要
目的 利用山茛菪碱具有稳定细胞膜和抗氧自由基损伤的作用 ,在低温保存期间用山茛菪碱对移植肝脏进行保护 ,从而改善移植肝脏的质量。方法 建立大鼠下腔静脉内分流法自体原位肝移植动物模型。动态观察肝脏氧自由基及酶学的变化。结果 实验组用山茛菪碱可减少再灌流后脂质过氧化物酶 (LPO)的增加 ,超氧化物歧化酶 (SOD)和谷胱甘肽过氧化物酶 (GSH Px)的下降 ,丙氨酸转氨酶 (ALT)乳酸脱氢酶 (LDH)的升高 ,与对照组比较 ,P <0 .0 1。结论 再灌流前应用山茛菪碱能减少再灌流后氧自由基的产生 ,可减轻缺血—再灌流对移植肝脏的损伤。
Objective To improve the quality of grafted liver, anisodamine was used to protect the grafted liver during the preservation of low temperature.Methods The model of orthotopic liver autotransplantation with intra inferior vana cava shunting method was set up. The changes of oxygen derived free radical and enzymes (LPO, SOD, GSH Px, ALT and LDH) were dynamically observed.Results Anisodamine could reduce the increase of LPO, ALT and LDH and the decrease of SOD and GSH Px significantly after reperfusion ( P < 0.01 ).Conclusions The use of anisodamine before reperfusion could reduce the production of oxygen derived free radical, therefore alleviate the injury of grafted liver caused by ischemia reperfusion.
出处
《中华器官移植杂志》
CAS
CSCD
2000年第2期86-88,共3页
Chinese Journal of Organ Transplantation
基金
辽宁省自然科学基金!96 2 2 80
